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Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in the articular cartilage on the equine third carpal bone in trained and untrained horses

Skioldebrand, E.; Ekman, S.; Heinegård, Dick LU and Hultenby, K. (2010) In Research in Veterinary Science 88(2). p.251-257
Abstract
The present study was designed to delineate the presence of COMP at the ultrastructural level comparing concentrations between two areas of articular cartilage from the equine third carpal bone, subjected to different loading, from trained and untrained horses. We also analyzed the fibril thickness of collagen type II in the same compartments and zones. Samples were collected from high load-bearing areas of the dorsal radial facet (intermittent high load) and an area of the palmar condyle (low constant load) in five non-trained and three trained young racehorses. The data show that COMP is much less abundant in the matrix in intermittent high loaded areas of articular cartilage from trained horses as compared to the untrained horses (p =... (More)
The present study was designed to delineate the presence of COMP at the ultrastructural level comparing concentrations between two areas of articular cartilage from the equine third carpal bone, subjected to different loading, from trained and untrained horses. We also analyzed the fibril thickness of collagen type II in the same compartments and zones. Samples were collected from high load-bearing areas of the dorsal radial facet (intermittent high load) and an area of the palmar condyle (low constant load) in five non-trained and three trained young racehorses. The data show that COMP is much less abundant in the matrix in intermittent high loaded areas of articular cartilage from trained horses as compared to the untrained horses (p = 0.036). On the other hand, the untrained horses often displayed a higher immunolabeling in loaded areas compared to unloaded areas, indicating that an adequate dynamic load promotes COMP synthesis and/or retention, while an excessive load may have an opposite effect. The collagen fibril diameter showed marked variation between individuals. The present study indicates that dynamic in vivo compression at high load and frequency lowers matrix content of COMP in the articular cartilage of the third carpal bone. It also indicates that the collagen network is influenced by mechanical load following by strenuous exercise. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Middle carpal bone, Load, COMP, Cartilage
in
Research in Veterinary Science
volume
88
issue
2
pages
251 - 257
publisher
Elsevier
external identifiers
  • wos:000275970100011
  • scopus:77149154344
ISSN
0034-5288
DOI
10.1016/j.rvsc.2009.07.011
language
English
LU publication?
yes
id
876287a2-eaa7-4977-9a7f-eed8112ed157 (old id 1587262)
date added to LUP
2010-04-22 15:48:08
date last changed
2018-05-29 10:58:23
@article{876287a2-eaa7-4977-9a7f-eed8112ed157,
  abstract     = {The present study was designed to delineate the presence of COMP at the ultrastructural level comparing concentrations between two areas of articular cartilage from the equine third carpal bone, subjected to different loading, from trained and untrained horses. We also analyzed the fibril thickness of collagen type II in the same compartments and zones. Samples were collected from high load-bearing areas of the dorsal radial facet (intermittent high load) and an area of the palmar condyle (low constant load) in five non-trained and three trained young racehorses. The data show that COMP is much less abundant in the matrix in intermittent high loaded areas of articular cartilage from trained horses as compared to the untrained horses (p = 0.036). On the other hand, the untrained horses often displayed a higher immunolabeling in loaded areas compared to unloaded areas, indicating that an adequate dynamic load promotes COMP synthesis and/or retention, while an excessive load may have an opposite effect. The collagen fibril diameter showed marked variation between individuals. The present study indicates that dynamic in vivo compression at high load and frequency lowers matrix content of COMP in the articular cartilage of the third carpal bone. It also indicates that the collagen network is influenced by mechanical load following by strenuous exercise. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Skioldebrand, E. and Ekman, S. and Heinegård, Dick and Hultenby, K.},
  issn         = {0034-5288},
  keyword      = {Middle carpal bone,Load,COMP,Cartilage},
  language     = {eng},
  number       = {2},
  pages        = {251--257},
  publisher    = {Elsevier},
  series       = {Research in Veterinary Science},
  title        = {Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in the articular cartilage on the equine third carpal bone in trained and untrained horses},
  url          = {http://dx.doi.org/10.1016/j.rvsc.2009.07.011},
  volume       = {88},
  year         = {2010},
}