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Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening

Dubicke, Aurelija; Fransson, Emma; Centini, Gabriele; Andersson, Eva; Bystrom, Birgitta; Malmström, Anders LU ; Petraglia, Felice; Sverremark-Ekstrom, Eva and Ekman-Ordeberg, Gunvor (2010) In Journal of Reproductive Immunology 84(2). p.176-185
Abstract
Cervical ripening is necessary for successful delivery. Since cytokines are believed to be involved in this process, the aim of this study was to investigate possible changes in the mRNA and protein expression of pro-inflammatory cytokines (interleukin (IL)-1 alpha, IL-1 beta, IL-12, IL-18) and anti-inflammatory cytokines (IL-4, IL-10, IL-13)in the human cervix during pregnancy, term and preterm labor. Cervical biopsies were taken from 59 women: 21 at preterm labor, 24 at term labor, 10 at term not in labor and 4 from non-pregnant women. mRNA was analyzed with real-time RT-PCR and protein expression and/or secretion with immunohistochemistry and ELISA. There was an upregulation of mRNA for IL-10, IL-13, IL-1 alpha and IL-1 beta in the... (More)
Cervical ripening is necessary for successful delivery. Since cytokines are believed to be involved in this process, the aim of this study was to investigate possible changes in the mRNA and protein expression of pro-inflammatory cytokines (interleukin (IL)-1 alpha, IL-1 beta, IL-12, IL-18) and anti-inflammatory cytokines (IL-4, IL-10, IL-13)in the human cervix during pregnancy, term and preterm labor. Cervical biopsies were taken from 59 women: 21 at preterm labor, 24 at term labor, 10 at term not in labor and 4 from non-pregnant women. mRNA was analyzed with real-time RT-PCR and protein expression and/or secretion with immunohistochemistry and ELISA. There was an upregulation of mRNA for IL-10, IL-13, IL-1 alpha and IL-1 beta in the laboring groups, while mRNA for IL-12 and IL-18 was downregulated. IL-4 mRNA was detected more frequently, while IL-12 mRNA expression was lower, in the preterm labor group than in the term labor group. The protein levels of IL-4 and IL-12 were lower and IL-18 tended to be higher in the labor groups, while IL-10 protein levels were unaffected by labor. IL-4 protein levels were significantly higher in the preterm subgroup with bacterial infection than in the non-infected group. IL-10 had higher expression in squamous epithelium at preterm labor than at term. In conclusion, the major changes in pro-inflammatory and anti-inflammatory cytokine mRNA and protein expression in cervix occur during the labor process irrespective of the length of gestation. Our results indicate that dysregulation of anti-inflammatory cytokines in the human cervix could be involved in the pathogenesis of preterm labor. (C) 2010 Elsevier Ireland Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Preterm birth, Cytokines, Cervical ripening, Cervix
in
Journal of Reproductive Immunology
volume
84
issue
2
pages
176 - 185
publisher
Elsevier
external identifiers
  • wos:000275965100008
  • scopus:77049093914
ISSN
1872-7603
DOI
10.1016/j.jri.2009.12.004
language
English
LU publication?
yes
id
67d1e748-3235-402c-8420-f49923b3ab97 (old id 1587732)
date added to LUP
2010-04-22 14:56:48
date last changed
2018-06-24 03:04:21
@article{67d1e748-3235-402c-8420-f49923b3ab97,
  abstract     = {Cervical ripening is necessary for successful delivery. Since cytokines are believed to be involved in this process, the aim of this study was to investigate possible changes in the mRNA and protein expression of pro-inflammatory cytokines (interleukin (IL)-1 alpha, IL-1 beta, IL-12, IL-18) and anti-inflammatory cytokines (IL-4, IL-10, IL-13)in the human cervix during pregnancy, term and preterm labor. Cervical biopsies were taken from 59 women: 21 at preterm labor, 24 at term labor, 10 at term not in labor and 4 from non-pregnant women. mRNA was analyzed with real-time RT-PCR and protein expression and/or secretion with immunohistochemistry and ELISA. There was an upregulation of mRNA for IL-10, IL-13, IL-1 alpha and IL-1 beta in the laboring groups, while mRNA for IL-12 and IL-18 was downregulated. IL-4 mRNA was detected more frequently, while IL-12 mRNA expression was lower, in the preterm labor group than in the term labor group. The protein levels of IL-4 and IL-12 were lower and IL-18 tended to be higher in the labor groups, while IL-10 protein levels were unaffected by labor. IL-4 protein levels were significantly higher in the preterm subgroup with bacterial infection than in the non-infected group. IL-10 had higher expression in squamous epithelium at preterm labor than at term. In conclusion, the major changes in pro-inflammatory and anti-inflammatory cytokine mRNA and protein expression in cervix occur during the labor process irrespective of the length of gestation. Our results indicate that dysregulation of anti-inflammatory cytokines in the human cervix could be involved in the pathogenesis of preterm labor. (C) 2010 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Dubicke, Aurelija and Fransson, Emma and Centini, Gabriele and Andersson, Eva and Bystrom, Birgitta and Malmström, Anders and Petraglia, Felice and Sverremark-Ekstrom, Eva and Ekman-Ordeberg, Gunvor},
  issn         = {1872-7603},
  keyword      = {Preterm birth,Cytokines,Cervical ripening,Cervix},
  language     = {eng},
  number       = {2},
  pages        = {176--185},
  publisher    = {Elsevier},
  series       = {Journal of Reproductive Immunology},
  title        = {Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening},
  url          = {http://dx.doi.org/10.1016/j.jri.2009.12.004},
  volume       = {84},
  year         = {2010},
}