Advanced

Feto-maternal interactions in pregnancies: Placental microparticles activate peripheral blood monocytes

Messerli, M.; May, K; Hansson, Stefan LU ; Schneider, H.; Holzgreve, W.; Hahn, S. and Rusterholz, C. (2010) In Placenta 31(2). p.106-112
Abstract
Normal pregnancy is associated with a systemic maternal inflammatory reaction, including the activation of peripheral blood monocytes. This reaction is exaggerated in pre-eclampsia, a severe placenta-dependent disorder of pregnancy specific to humans It has been suggested that placental syncytiotrophoblast membrane microparticles (STBM), which are released into the peripheral blood, may contribute to the maternal response The aim of this study was to investigate the inflammatory properties of STBM generated by four different approaches on primary human monocytes in vitro. Cellular viability, phenotype and functional response were analysed STBM isolated by mechanical dissection and STBM generated from villous explant Cultures incubated in... (More)
Normal pregnancy is associated with a systemic maternal inflammatory reaction, including the activation of peripheral blood monocytes. This reaction is exaggerated in pre-eclampsia, a severe placenta-dependent disorder of pregnancy specific to humans It has been suggested that placental syncytiotrophoblast membrane microparticles (STBM), which are released into the peripheral blood, may contribute to the maternal response The aim of this study was to investigate the inflammatory properties of STBM generated by four different approaches on primary human monocytes in vitro. Cellular viability, phenotype and functional response were analysed STBM isolated by mechanical dissection and STBM generated from villous explant Cultures incubated in hypoxic conditions had only minor influences on the monocytic phenotype and failed to induce a proinflammatory response By contrast, STBM washed from the maternal side of a placental cotyledon and STBM shed by explants cultured in air up-regulated cell surface expression of the adhesion molecule CD54 and induced the production of interleukin (IL)-8, IL-6 and IL-1 beta. Cytokine production was time- and dose-dependent. Our study, therefore, suggests that monocyte activation in normal pregnancy and pre-eclampsia may be induced by STBM released by the placenta. The higher amounts of STBM circulating in maternal blood in pre-eclampsia might lead to the excessive maternal inflammatory reaction. (C) 2009 Elsevier Ltd All rights reserved (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Feto-maternal, Monocytes, Pregnancy, Placental microparticles, interactions
in
Placenta
volume
31
issue
2
pages
106 - 112
publisher
W B Saunders
external identifiers
  • wos:000275010700005
  • scopus:74849085113
ISSN
1532-3102
DOI
10.1016/j.placenta.2009.11.011
language
English
LU publication?
yes
id
d2e863d4-5acc-40b6-8d66-2f40af679447 (old id 1589358)
date added to LUP
2010-04-20 12:30:51
date last changed
2018-05-29 12:23:33
@article{d2e863d4-5acc-40b6-8d66-2f40af679447,
  abstract     = {Normal pregnancy is associated with a systemic maternal inflammatory reaction, including the activation of peripheral blood monocytes. This reaction is exaggerated in pre-eclampsia, a severe placenta-dependent disorder of pregnancy specific to humans It has been suggested that placental syncytiotrophoblast membrane microparticles (STBM), which are released into the peripheral blood, may contribute to the maternal response The aim of this study was to investigate the inflammatory properties of STBM generated by four different approaches on primary human monocytes in vitro. Cellular viability, phenotype and functional response were analysed STBM isolated by mechanical dissection and STBM generated from villous explant Cultures incubated in hypoxic conditions had only minor influences on the monocytic phenotype and failed to induce a proinflammatory response By contrast, STBM washed from the maternal side of a placental cotyledon and STBM shed by explants cultured in air up-regulated cell surface expression of the adhesion molecule CD54 and induced the production of interleukin (IL)-8, IL-6 and IL-1 beta. Cytokine production was time- and dose-dependent. Our study, therefore, suggests that monocyte activation in normal pregnancy and pre-eclampsia may be induced by STBM released by the placenta. The higher amounts of STBM circulating in maternal blood in pre-eclampsia might lead to the excessive maternal inflammatory reaction. (C) 2009 Elsevier Ltd All rights reserved},
  author       = {Messerli, M. and May, K and Hansson, Stefan and Schneider, H. and Holzgreve, W. and Hahn, S. and Rusterholz, C.},
  issn         = {1532-3102},
  keyword      = {Feto-maternal,Monocytes,Pregnancy,Placental microparticles,interactions},
  language     = {eng},
  number       = {2},
  pages        = {106--112},
  publisher    = {W B Saunders},
  series       = {Placenta},
  title        = {Feto-maternal interactions in pregnancies: Placental microparticles activate peripheral blood monocytes},
  url          = {http://dx.doi.org/10.1016/j.placenta.2009.11.011},
  volume       = {31},
  year         = {2010},
}