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alpha-synuclein antibodies recognize a protein present at lower levels in the CSF of patients with dementia with Lewy bodies

Ballard, Clive ; Jones, Emma L. ; Londos, Elisabet LU ; Minthon, Lennart LU ; Francis, Paul and Aarsland, Dag (2010) In International Psychogeriatrics 22(2). p.321-327
Abstract
Background: Dementia with Lewy bodies (DLB) accounts for 15-20%, of the millions of people worldwide with dementia. Accurate diagnosis is essential to avoid harm and optimize clinical management. There is therefore an urgent need to identify reliable biomarkers. Methods: Mass spectrometry was used to determine the specificity of antibody alpha-synuclein (211) for alpha-synuclein. Using gel electrophoresis we measured protein levels detected by alpha-synuclein specific antibodies in the cerebrospinal fluid (CSF) of DLB patients and compared them to age matched controls. Results: A 24 kDa band was detected using alpha-synuclein specific antibodies which was significantly reduced in the CSF of DLB patients compared to age matched controls (p... (More)
Background: Dementia with Lewy bodies (DLB) accounts for 15-20%, of the millions of people worldwide with dementia. Accurate diagnosis is essential to avoid harm and optimize clinical management. There is therefore an urgent need to identify reliable biomarkers. Methods: Mass spectrometry was used to determine the specificity of antibody alpha-synuclein (211) for alpha-synuclein. Using gel electrophoresis we measured protein levels detected by alpha-synuclein specific antibodies in the cerebrospinal fluid (CSF) of DLB patients and compared them to age matched controls. Results: A 24 kDa band was detected using alpha-synuclein specific antibodies which was significantly reduced in the CSF of DLB patients compared to age matched controls (p < 0.05). Further analysis confirmed that even DLB patients with mild dementia showed significant reductions in this protein in comparison to controls. Conclusions: The current study emphasizes the necessity for further studies of CSF alpha-synuclein as a biomarker of DLB and extends our previous knowledge by establishing a potential relationship between alpha-synuclein and the severity of cognitive impairment. The identification of this 24 kDa protein is the next important step in these studies. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biomarkers, cognitive decline
in
International Psychogeriatrics
volume
22
issue
2
pages
321 - 327
publisher
Cambridge University Press
external identifiers
  • wos:000275132800018
  • scopus:77950567700
  • pmid:19747426
ISSN
1741-203X
DOI
10.1017/S1041610209991049
language
English
LU publication?
yes
id
9d7d3c1c-2c90-4303-be79-f489dc2127c4 (old id 1589563)
date added to LUP
2016-04-01 10:21:57
date last changed
2022-04-27 21:24:02
@article{9d7d3c1c-2c90-4303-be79-f489dc2127c4,
  abstract     = {{Background: Dementia with Lewy bodies (DLB) accounts for 15-20%, of the millions of people worldwide with dementia. Accurate diagnosis is essential to avoid harm and optimize clinical management. There is therefore an urgent need to identify reliable biomarkers. Methods: Mass spectrometry was used to determine the specificity of antibody alpha-synuclein (211) for alpha-synuclein. Using gel electrophoresis we measured protein levels detected by alpha-synuclein specific antibodies in the cerebrospinal fluid (CSF) of DLB patients and compared them to age matched controls. Results: A 24 kDa band was detected using alpha-synuclein specific antibodies which was significantly reduced in the CSF of DLB patients compared to age matched controls (p &lt; 0.05). Further analysis confirmed that even DLB patients with mild dementia showed significant reductions in this protein in comparison to controls. Conclusions: The current study emphasizes the necessity for further studies of CSF alpha-synuclein as a biomarker of DLB and extends our previous knowledge by establishing a potential relationship between alpha-synuclein and the severity of cognitive impairment. The identification of this 24 kDa protein is the next important step in these studies.}},
  author       = {{Ballard, Clive and Jones, Emma L. and Londos, Elisabet and Minthon, Lennart and Francis, Paul and Aarsland, Dag}},
  issn         = {{1741-203X}},
  keywords     = {{biomarkers; cognitive decline}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{321--327}},
  publisher    = {{Cambridge University Press}},
  series       = {{International Psychogeriatrics}},
  title        = {{alpha-synuclein antibodies recognize a protein present at lower levels in the CSF of patients with dementia with Lewy bodies}},
  url          = {{http://dx.doi.org/10.1017/S1041610209991049}},
  doi          = {{10.1017/S1041610209991049}},
  volume       = {{22}},
  year         = {{2010}},
}