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Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements.

Schartau, Marcus LU ; Kröger, Ronald LU and Sjögreen, Bodil LU (2010) In Cytotechnology 62. p.167-174
Abstract
Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to... (More)
Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to temperature and nutrient fluctuations. Teleost lenses are structurally more robust and can be excised from the eye without disturbing form or function. The technique is developed for short-term culturing (3 h), however, the lenses appear viable for at least 24 h and longer culturing may be possible. The technique is resistant to small variations in osmolarity and yields quantitative datasets for further analyses and statistical treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Optical measurements, Teleost, Lens, Organ culture
in
Cytotechnology
volume
62
pages
167 - 174
publisher
Springer
external identifiers
  • wos:000279053700009
  • scopus:77955717398
ISSN
0920-9069
DOI
10.1007/s10616-010-9268-y
language
English
LU publication?
yes
id
487061bf-a41b-4118-8679-407aff01c570 (old id 1595109)
date added to LUP
2010-05-17 12:48:54
date last changed
2018-05-29 12:23:04
@article{487061bf-a41b-4118-8679-407aff01c570,
  abstract     = {Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to temperature and nutrient fluctuations. Teleost lenses are structurally more robust and can be excised from the eye without disturbing form or function. The technique is developed for short-term culturing (3 h), however, the lenses appear viable for at least 24 h and longer culturing may be possible. The technique is resistant to small variations in osmolarity and yields quantitative datasets for further analyses and statistical treatment.},
  author       = {Schartau, Marcus and Kröger, Ronald and Sjögreen, Bodil},
  issn         = {0920-9069},
  keyword      = {Optical measurements,Teleost,Lens,Organ culture},
  language     = {eng},
  pages        = {167--174},
  publisher    = {Springer},
  series       = {Cytotechnology},
  title        = {Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements.},
  url          = {http://dx.doi.org/10.1007/s10616-010-9268-y},
  volume       = {62},
  year         = {2010},
}