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Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch

Lindgren, Gustav LU ; Wennerberg, Johan LU and Ekblad, Lars LU (2017) In Laryngoscope 2(4). p.194-198
Abstract

OBJECTIVES: The existence of circulating tumor cells has emerged as an important factor for prognosis and survival. The CellSearch method is the only circulating tumor cell detection method approved by the US Food and Drug Administration for clinical use. It relies on the detection of EpCAM (epithelial cell adhesion molecule) and is approved for colon cancer, breast cancer and prostate cancer. We now investigated whether CellSearch can be used to quantify circulating tumor cells in head and neck squamous cell cancer.

STUDY DESIGN AND METHODS: In this study, we investigated the expression of EpCAM in 12 head and neck squamous cell cancer cell lines using Western blot and how this affected their detectability with CellSearch in... (More)

OBJECTIVES: The existence of circulating tumor cells has emerged as an important factor for prognosis and survival. The CellSearch method is the only circulating tumor cell detection method approved by the US Food and Drug Administration for clinical use. It relies on the detection of EpCAM (epithelial cell adhesion molecule) and is approved for colon cancer, breast cancer and prostate cancer. We now investigated whether CellSearch can be used to quantify circulating tumor cells in head and neck squamous cell cancer.

STUDY DESIGN AND METHODS: In this study, we investigated the expression of EpCAM in 12 head and neck squamous cell cancer cell lines using Western blot and how this affected their detectability with CellSearch in peripheral blood.

RESULTS: We found a great variation in the expression of EpCAM between our head and neck squamous cell cancer cell lines. This was accompanied by variations in counting efficiency.

CONCLUSION: We suggest that for reliable quantification of circulating tumor cells in blood from patients with head and neck squamous cell cancer cell, an epitope independent method is preferable.

LEVEL OF EVIDENCE: NA.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Laryngoscope
volume
2
issue
4
pages
5 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000407941600008
ISSN
0023-852X
DOI
10.1002/lio2.83
language
English
LU publication?
yes
id
15a806cf-248b-4919-a2d2-d41fc6acefc6
date added to LUP
2017-10-06 16:18:34
date last changed
2018-01-16 13:21:37
@article{15a806cf-248b-4919-a2d2-d41fc6acefc6,
  abstract     = {<p>OBJECTIVES: The existence of circulating tumor cells has emerged as an important factor for prognosis and survival. The CellSearch method is the only circulating tumor cell detection method approved by the US Food and Drug Administration for clinical use. It relies on the detection of EpCAM (epithelial cell adhesion molecule) and is approved for colon cancer, breast cancer and prostate cancer. We now investigated whether CellSearch can be used to quantify circulating tumor cells in head and neck squamous cell cancer.</p><p>STUDY DESIGN AND METHODS: In this study, we investigated the expression of EpCAM in 12 head and neck squamous cell cancer cell lines using Western blot and how this affected their detectability with CellSearch in peripheral blood.</p><p>RESULTS: We found a great variation in the expression of EpCAM between our head and neck squamous cell cancer cell lines. This was accompanied by variations in counting efficiency.</p><p>CONCLUSION: We suggest that for reliable quantification of circulating tumor cells in blood from patients with head and neck squamous cell cancer cell, an epitope independent method is preferable.</p><p>LEVEL OF EVIDENCE: NA.</p>},
  author       = {Lindgren, Gustav and Wennerberg, Johan and Ekblad, Lars},
  issn         = {0023-852X},
  language     = {eng},
  number       = {4},
  pages        = {194--198},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Laryngoscope},
  title        = {Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch},
  url          = {http://dx.doi.org/10.1002/lio2.83},
  volume       = {2},
  year         = {2017},
}