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Immune Suppression by Cyclosporin A Inhibits Phytohemagglutinin-induced Precocious Gut Maturation in Suckling Rats

Prykhodko, Olena LU ; Pierzynowski, Stefan LU and Weström, Björn LU (2010) In Journal of Pediatric Gastroenterology and Nutrition - Jpgn 50(5). p.473-480
Abstract
Objectives: Enteral exposure to the lectin phytohemagglutinin (PHA) provokes precocious gut maturation in suckling rats coinciding with an early expansion of intestinal mucosal T and B lymphocytes. Here, the role of the immune system in neonatal gut growth and maturation was further studied. Materials and Methods: The effects of immunosuppression by cyclosporine A (CyA), 7.5 mu g/g of body weight, injected 12 hours before and then daily after the intragastric gavage of PHA, 100 mu g/g body weight, to 14-day-old suckling rats were studied after 4 and 12 hours and later after 72 hours. Results: At 4 hours after PHA feeding, an early rapid increase in the intestinal levels of the proinflammatory cytokines interleukin-6, interleukin-1 beta,... (More)
Objectives: Enteral exposure to the lectin phytohemagglutinin (PHA) provokes precocious gut maturation in suckling rats coinciding with an early expansion of intestinal mucosal T and B lymphocytes. Here, the role of the immune system in neonatal gut growth and maturation was further studied. Materials and Methods: The effects of immunosuppression by cyclosporine A (CyA), 7.5 mu g/g of body weight, injected 12 hours before and then daily after the intragastric gavage of PHA, 100 mu g/g body weight, to 14-day-old suckling rats were studied after 4 and 12 hours and later after 72 hours. Results: At 4 hours after PHA feeding, an early rapid increase in the intestinal levels of the proinflammatory cytokines interleukin-6, interleukin-1 beta, and tumor necrosis factor was obtained, and the CyA treatment did not prevent the temporary PHA-induced intestinal disturbance seen at 12 hours. Later, at 72 hours after PHA gavage the CyA treatment significantly counteracted the PHA-induced gut changes with a decrease in small intestinal growth, a delay in the appearance of adult-phenotype enterocytes in the distal small intestinal, and total inhibition of the PHA-induced pancreas development. Additionally, the increase in plasma level of the acute phase protein, haptoglobin, after PHA feeding was dampened by CyA. Conclusions: The results indicate that proinflammatory cytokines are involved in the early recruitment of lymphocytes to the gut after PHA challenge, and that the ensuing precocious gut maturation is dependent on activation of the immune system, presumably T cells, in suckling rats. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proinflammatory cytokines, haptoglobin, pancreas, small intestine
in
Journal of Pediatric Gastroenterology and Nutrition - Jpgn
volume
50
issue
5
pages
473 - 480
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000277037800002
  • scopus:77951864515
ISSN
1536-4801
DOI
10.1097/MPG.0b013e3181b47787
language
English
LU publication?
yes
id
704f7a9b-5ca9-4175-940e-19928497602e (old id 1601090)
date added to LUP
2010-05-20 10:53:13
date last changed
2018-06-10 03:23:49
@article{704f7a9b-5ca9-4175-940e-19928497602e,
  abstract     = {Objectives: Enteral exposure to the lectin phytohemagglutinin (PHA) provokes precocious gut maturation in suckling rats coinciding with an early expansion of intestinal mucosal T and B lymphocytes. Here, the role of the immune system in neonatal gut growth and maturation was further studied. Materials and Methods: The effects of immunosuppression by cyclosporine A (CyA), 7.5 mu g/g of body weight, injected 12 hours before and then daily after the intragastric gavage of PHA, 100 mu g/g body weight, to 14-day-old suckling rats were studied after 4 and 12 hours and later after 72 hours. Results: At 4 hours after PHA feeding, an early rapid increase in the intestinal levels of the proinflammatory cytokines interleukin-6, interleukin-1 beta, and tumor necrosis factor was obtained, and the CyA treatment did not prevent the temporary PHA-induced intestinal disturbance seen at 12 hours. Later, at 72 hours after PHA gavage the CyA treatment significantly counteracted the PHA-induced gut changes with a decrease in small intestinal growth, a delay in the appearance of adult-phenotype enterocytes in the distal small intestinal, and total inhibition of the PHA-induced pancreas development. Additionally, the increase in plasma level of the acute phase protein, haptoglobin, after PHA feeding was dampened by CyA. Conclusions: The results indicate that proinflammatory cytokines are involved in the early recruitment of lymphocytes to the gut after PHA challenge, and that the ensuing precocious gut maturation is dependent on activation of the immune system, presumably T cells, in suckling rats.},
  author       = {Prykhodko, Olena and Pierzynowski, Stefan and Weström, Björn},
  issn         = {1536-4801},
  keyword      = {proinflammatory cytokines,haptoglobin,pancreas,small intestine},
  language     = {eng},
  number       = {5},
  pages        = {473--480},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Pediatric Gastroenterology and Nutrition - Jpgn},
  title        = {Immune Suppression by Cyclosporin A Inhibits Phytohemagglutinin-induced Precocious Gut Maturation in Suckling Rats},
  url          = {http://dx.doi.org/10.1097/MPG.0b013e3181b47787},
  volume       = {50},
  year         = {2010},
}