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Fusion of Nonionic Vesicles

Bulut, Sanja; Zackrisson Oskolkova, Malin LU ; Schweins, Ralf; Wennerström, Håkan LU and Olsson, Ulf LU (2010) In Langmuir 26(8). p.5421-5427
Abstract
We present an experimental study of vesicle fusion using light and neutron scattering to monitor fusion events. Vesicles are reproducibly formed with an extrusion procedure using an single amphiphile triethylene glycol mono-n-decyl ether in water. They show long-term stability for temperatures around 20 C, but at temperatures above 26 C we observe an increase in the scattered intensity due to fusion. The system is unusually well suited for the study of basic mechanisms of vesicle fusion. The vesicles are flexible with a bending rigidity of only a few k(H)T. The monolayer spontaneous curvature, Ho, depends strongly on temperature in a known way and is thus tunable. For temperatures where H-0 > 0 vesicles tyre long-term stable, while in... (More)
We present an experimental study of vesicle fusion using light and neutron scattering to monitor fusion events. Vesicles are reproducibly formed with an extrusion procedure using an single amphiphile triethylene glycol mono-n-decyl ether in water. They show long-term stability for temperatures around 20 C, but at temperatures above 26 C we observe an increase in the scattered intensity due to fusion. The system is unusually well suited for the study of basic mechanisms of vesicle fusion. The vesicles are flexible with a bending rigidity of only a few k(H)T. The monolayer spontaneous curvature, Ho, depends strongly on temperature in a known way and is thus tunable. For temperatures where H-0 > 0 vesicles tyre long-term stable, while in the range H-0 < 0 the fusion rate increases the more negative the Spontaneous curvature Through a quantitative;analysis of the fusion rate we arrive tit a barrier to fusion changing from 15 k(B)T at T = 26 degrees C to 10k(H) T at T = 35 degrees C. These results are compatible with the theoretical predictions using the stalk model of vesicle fusion. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
26
issue
8
pages
5421 - 5427
publisher
The American Chemical Society
external identifiers
  • wos:000276562300021
  • scopus:77950952783
ISSN
0743-7463
DOI
10.1021/la903877f
language
English
LU publication?
yes
id
95516180-9a03-4c19-84fc-e14d6834023f (old id 1603830)
date added to LUP
2010-05-17 13:38:37
date last changed
2018-05-29 10:14:28
@article{95516180-9a03-4c19-84fc-e14d6834023f,
  abstract     = {We present an experimental study of vesicle fusion using light and neutron scattering to monitor fusion events. Vesicles are reproducibly formed with an extrusion procedure using an single amphiphile triethylene glycol mono-n-decyl ether in water. They show long-term stability for temperatures around 20 C, but at temperatures above 26 C we observe an increase in the scattered intensity due to fusion. The system is unusually well suited for the study of basic mechanisms of vesicle fusion. The vesicles are flexible with a bending rigidity of only a few k(H)T. The monolayer spontaneous curvature, Ho, depends strongly on temperature in a known way and is thus tunable. For temperatures where H-0 &gt; 0 vesicles tyre long-term stable, while in the range H-0 &lt; 0 the fusion rate increases the more negative the Spontaneous curvature Through a quantitative;analysis of the fusion rate we arrive tit a barrier to fusion changing from 15 k(B)T at T = 26 degrees C to 10k(H) T at T = 35 degrees C. These results are compatible with the theoretical predictions using the stalk model of vesicle fusion.},
  author       = {Bulut, Sanja and Zackrisson Oskolkova, Malin and Schweins, Ralf and Wennerström, Håkan and Olsson, Ulf},
  issn         = {0743-7463},
  language     = {eng},
  number       = {8},
  pages        = {5421--5427},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Fusion of Nonionic Vesicles},
  url          = {http://dx.doi.org/10.1021/la903877f},
  volume       = {26},
  year         = {2010},
}