Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

G protein-coupled estrogen receptor 1 (GPER, GPR 30) in normal human endometrium and early pregnancy decidua.

Kolkova, Zuzana LU ; Casslén, Vera LU ; Ehinger, Anna LU orcid ; Hansson, Stefan LU orcid and Casslén, Bertil LU (2010) In Molecular Human Reproduction 16(10). p.743-751
Abstract
The recently identified trans-membrane G protein-coupled estrogen receptor 1 (GPER, GPR30) has been implicated in rapid non-genomic effects of estrogens. This focusses on expression and localization of GPER mRNA and protein in normal cyclic endometrium and early pregnancy decidua. Real-time PCR, Western blotting, in situ hybridization, and immuno-histochemistry were used. Endometrial expression of GPER mRNA was lower in the secretory phase than in the proliferative phase , and even lower in the decidua. The expression pattern was similar to that of ERalpha mRNA, but different from that of ERss mRNA. Western blot detected GPER protein as a 54 kDa band in all endometrial and decidual samples. In contrast to the mRNA, GPER protein did not... (More)
The recently identified trans-membrane G protein-coupled estrogen receptor 1 (GPER, GPR30) has been implicated in rapid non-genomic effects of estrogens. This focusses on expression and localization of GPER mRNA and protein in normal cyclic endometrium and early pregnancy decidua. Real-time PCR, Western blotting, in situ hybridization, and immuno-histochemistry were used. Endometrial expression of GPER mRNA was lower in the secretory phase than in the proliferative phase , and even lower in the decidua. The expression pattern was similar to that of ERalpha mRNA, but different from that of ERss mRNA. Western blot detected GPER protein as a 54 kDa band in all endometrial and decidual samples. In contrast to the mRNA, GPER protein did not show cyclic variations. Apparently, a lower amount of mRNA is sufficient to maintain protein levels in the secretory phase. GPER mRNA was predominantly localized in the epithelium of mid and late proliferative phase endometrium, whereas expression in early proliferative and secretory glands could not be distinguished from the diffuse stromal signal, which was present throughout the cycle. Immuno-staining for GPER was stronger in glandular and luminal epithelium than in the stroma throughout the cycle. The cyclic variations of GPER mRNA obviously relate to strong epithelial expression in the proliferative phase, and the expression pattern suggests regulation by ovarian steroids. GPER protein is present in endometrial tissue throughout the cycle, and the epithelial localization suggests potential functions during sperm migration at midcycle as well as decidualization and blastocyst implantation in the mid-secretory phase. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ER beta, ER alpha, in situ hybridization, membrane estrogen receptor, menstrual cycle, physiologic hormone concentrations
in
Molecular Human Reproduction
volume
16
issue
10
pages
743 - 751
publisher
Oxford University Press
external identifiers
  • wos:000281949400004
  • pmid:20508064
  • scopus:77956793023
ISSN
1460-2407
DOI
10.1093/molehr/gaq043
language
English
LU publication?
yes
id
3beaaadb-30b9-43ee-b8c4-2c72fb28c114 (old id 1609805)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20508064?dopt=Abstract
date added to LUP
2016-04-01 10:10:56
date last changed
2022-04-12 02:50:39
@article{3beaaadb-30b9-43ee-b8c4-2c72fb28c114,
  abstract     = {{The recently identified trans-membrane G protein-coupled estrogen receptor 1 (GPER, GPR30) has been implicated in rapid non-genomic effects of estrogens. This focusses on expression and localization of GPER mRNA and protein in normal cyclic endometrium and early pregnancy decidua. Real-time PCR, Western blotting, in situ hybridization, and immuno-histochemistry were used. Endometrial expression of GPER mRNA was lower in the secretory phase than in the proliferative phase , and even lower in the decidua. The expression pattern was similar to that of ERalpha mRNA, but different from that of ERss mRNA. Western blot detected GPER protein as a 54 kDa band in all endometrial and decidual samples. In contrast to the mRNA, GPER protein did not show cyclic variations. Apparently, a lower amount of mRNA is sufficient to maintain protein levels in the secretory phase. GPER mRNA was predominantly localized in the epithelium of mid and late proliferative phase endometrium, whereas expression in early proliferative and secretory glands could not be distinguished from the diffuse stromal signal, which was present throughout the cycle. Immuno-staining for GPER was stronger in glandular and luminal epithelium than in the stroma throughout the cycle. The cyclic variations of GPER mRNA obviously relate to strong epithelial expression in the proliferative phase, and the expression pattern suggests regulation by ovarian steroids. GPER protein is present in endometrial tissue throughout the cycle, and the epithelial localization suggests potential functions during sperm migration at midcycle as well as decidualization and blastocyst implantation in the mid-secretory phase.}},
  author       = {{Kolkova, Zuzana and Casslén, Vera and Ehinger, Anna and Hansson, Stefan and Casslén, Bertil}},
  issn         = {{1460-2407}},
  keywords     = {{ER beta; ER alpha; in situ hybridization; membrane estrogen receptor; menstrual cycle; physiologic hormone concentrations}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{743--751}},
  publisher    = {{Oxford University Press}},
  series       = {{Molecular Human Reproduction}},
  title        = {{G protein-coupled estrogen receptor 1 (GPER, GPR 30) in normal human endometrium and early pregnancy decidua.}},
  url          = {{http://dx.doi.org/10.1093/molehr/gaq043}},
  doi          = {{10.1093/molehr/gaq043}},
  volume       = {{16}},
  year         = {{2010}},
}