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Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus

Pagels, Martin; Fuchs, Stephan; Pane-Farre, Jan; Kohler, Christian; Menschner, Leonhard; Hecker, Michael; McNamarra, Peter J.; Bauer, Mikael LU ; von Wachenfeldt, Claes LU and Liebeke, Manuel, et al. (2010) In Molecular Microbiology 76(5). p.1142-1161
Abstract
P>An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of S. aureus binds to this inverted repeat. The binding sequence - TTGTGAAW(4)TTCACAA - is highly conserved in S. aureus. Rex binding to this sequence leads to the repression of genes located downstream. The binding activity of Rex is enhanced by NAD+ while NADH, which competes with NAD+ for Rex binding, decreases the activity of Rex. The impact of Rex on global protein synthesis and on the activity of fermentation pathways under aerobic and... (More)
P>An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of S. aureus binds to this inverted repeat. The binding sequence - TTGTGAAW(4)TTCACAA - is highly conserved in S. aureus. Rex binding to this sequence leads to the repression of genes located downstream. The binding activity of Rex is enhanced by NAD+ while NADH, which competes with NAD+ for Rex binding, decreases the activity of Rex. The impact of Rex on global protein synthesis and on the activity of fermentation pathways under aerobic and anaerobic conditions was analysed by using a rex-deficient strain. A direct regulatory effect of Rex on the expression of pathways that lead to anaerobic NAD+ regeneration, such as lactate, formate and ethanol formation, nitrate respiration, and ATP synthesis, is verified. Rex can be considered a central regulator of anaerobic metabolism in S. aureus. Since the activity of lactate dehydrogenase enables S. aureus to resist NO stress and thus the innate immune response, our data suggest that deactivation of Rex is a prerequisite for this phenomenon. (Less)
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publication status
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subject
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Molecular Microbiology
volume
76
issue
5
pages
1142 - 1161
publisher
Wiley-Blackwell
external identifiers
  • wos:000277981100007
  • scopus:77952821682
ISSN
1365-2958
DOI
10.1111/j.1365-2958.2010.07105.x
language
English
LU publication?
yes
id
dc76ecb1-4b57-4b94-aa26-2b761cf90c3d (old id 1616705)
date added to LUP
2010-06-22 14:02:54
date last changed
2018-07-01 03:15:35
@article{dc76ecb1-4b57-4b94-aa26-2b761cf90c3d,
  abstract     = {P>An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of S. aureus binds to this inverted repeat. The binding sequence - TTGTGAAW(4)TTCACAA - is highly conserved in S. aureus. Rex binding to this sequence leads to the repression of genes located downstream. The binding activity of Rex is enhanced by NAD+ while NADH, which competes with NAD+ for Rex binding, decreases the activity of Rex. The impact of Rex on global protein synthesis and on the activity of fermentation pathways under aerobic and anaerobic conditions was analysed by using a rex-deficient strain. A direct regulatory effect of Rex on the expression of pathways that lead to anaerobic NAD+ regeneration, such as lactate, formate and ethanol formation, nitrate respiration, and ATP synthesis, is verified. Rex can be considered a central regulator of anaerobic metabolism in S. aureus. Since the activity of lactate dehydrogenase enables S. aureus to resist NO stress and thus the innate immune response, our data suggest that deactivation of Rex is a prerequisite for this phenomenon.},
  author       = {Pagels, Martin and Fuchs, Stephan and Pane-Farre, Jan and Kohler, Christian and Menschner, Leonhard and Hecker, Michael and McNamarra, Peter J. and Bauer, Mikael and von Wachenfeldt, Claes and Liebeke, Manuel and Lalk, Michael and Sander, Gunnar and von Eiff, Christof and Proctor, Richard A. and Engelmann, Susanne},
  issn         = {1365-2958},
  language     = {eng},
  number       = {5},
  pages        = {1142--1161},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2010.07105.x},
  volume       = {76},
  year         = {2010},
}