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Composition and Systemic Immune Activity of the Polysaccharides from an Herbal Tea (Lycopus lucidus Turcz)

Yang, Xingbin; You, Liwen LU ; Tian, Lingmin and Zhao, Yan (2010) In Journal of Agricultural and Food Chemistry 58(10). p.6075-6080
Abstract
This study was undertaken to characterize the water-soluble polysaccharides isolated from an herbal tea, the leaves of L. lucidus Turcz. HPLC analysis showed that L. lucidus polysaccharides (LLPs) were mainly composed of galactose (50.1 mol %), followed by galacturonic acid (14.2 mol %), accounting for 64.3 mol % of all quantitative nine monosaccharides. Furthermore, we evaluated the systemic immunological efficacy of LLPs in mice. Mice were intragastrically administered once daily with low-dose (50 mg/kg), intermediate-dose (100 mg/kg), and high-dose (300 mg/kg) of LLPs, respectively, for 30 consecutive days. In comparison with vehicle, LLPs significantly enhanced the plaque-forming cells (PFCs), and serum hemolysin level, and... (More)
This study was undertaken to characterize the water-soluble polysaccharides isolated from an herbal tea, the leaves of L. lucidus Turcz. HPLC analysis showed that L. lucidus polysaccharides (LLPs) were mainly composed of galactose (50.1 mol %), followed by galacturonic acid (14.2 mol %), accounting for 64.3 mol % of all quantitative nine monosaccharides. Furthermore, we evaluated the systemic immunological efficacy of LLPs in mice. Mice were intragastrically administered once daily with low-dose (50 mg/kg), intermediate-dose (100 mg/kg), and high-dose (300 mg/kg) of LLPs, respectively, for 30 consecutive days. In comparison with vehicle, LLPs significantly enhanced the plaque-forming cells (PFCs), and serum hemolysin level, and delayed-type hypersensitivity (DTH) in response to sheep red blood cells (SRBC) in a dose-dependent manner (p<0.01). In LLPs-treated mice, phagocytosis capacity and concanavalin A-induced spleenocyte proliferation were remarkably increased (p < 0.05). The intermediate- and high-dose of LLPs also caused a significant increase in the indices of thymus and spleen organs of mice (p < 0.05). This suggests that the polysaccharides derived from the tea leaves of L. lucidus improves the immune system and might be regarded as a biological response modifier. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polysaccharides, L. lucidus Turcz, tea leaves, HPLC, immunomodulatory, activity
in
Journal of Agricultural and Food Chemistry
volume
58
issue
10
pages
6075 - 6080
publisher
The American Chemical Society
external identifiers
  • wos:000277721900025
  • scopus:77952603162
ISSN
0021-8561
DOI
10.1021/jf101061y
language
English
LU publication?
yes
id
9b0ed841-f3c5-4a4d-bc97-3c74283b6f00 (old id 1617422)
date added to LUP
2010-06-23 13:10:44
date last changed
2018-05-29 10:20:03
@article{9b0ed841-f3c5-4a4d-bc97-3c74283b6f00,
  abstract     = {This study was undertaken to characterize the water-soluble polysaccharides isolated from an herbal tea, the leaves of L. lucidus Turcz. HPLC analysis showed that L. lucidus polysaccharides (LLPs) were mainly composed of galactose (50.1 mol %), followed by galacturonic acid (14.2 mol %), accounting for 64.3 mol % of all quantitative nine monosaccharides. Furthermore, we evaluated the systemic immunological efficacy of LLPs in mice. Mice were intragastrically administered once daily with low-dose (50 mg/kg), intermediate-dose (100 mg/kg), and high-dose (300 mg/kg) of LLPs, respectively, for 30 consecutive days. In comparison with vehicle, LLPs significantly enhanced the plaque-forming cells (PFCs), and serum hemolysin level, and delayed-type hypersensitivity (DTH) in response to sheep red blood cells (SRBC) in a dose-dependent manner (p&lt;0.01). In LLPs-treated mice, phagocytosis capacity and concanavalin A-induced spleenocyte proliferation were remarkably increased (p &lt; 0.05). The intermediate- and high-dose of LLPs also caused a significant increase in the indices of thymus and spleen organs of mice (p &lt; 0.05). This suggests that the polysaccharides derived from the tea leaves of L. lucidus improves the immune system and might be regarded as a biological response modifier.},
  author       = {Yang, Xingbin and You, Liwen and Tian, Lingmin and Zhao, Yan},
  issn         = {0021-8561},
  keyword      = {polysaccharides,L. lucidus Turcz,tea leaves,HPLC,immunomodulatory,activity},
  language     = {eng},
  number       = {10},
  pages        = {6075--6080},
  publisher    = {The American Chemical Society},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Composition and Systemic Immune Activity of the Polysaccharides from an Herbal Tea (Lycopus lucidus Turcz)},
  url          = {http://dx.doi.org/10.1021/jf101061y},
  volume       = {58},
  year         = {2010},
}