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Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads

Mbatia, Betty LU ; Adlercreutz, Dietlind LU ; Adlercreutz, Patrick LU ; Mahadhy, Ally LU ; Mulaa, Francis and Mattiasson, Bo LU (2010) In Process Biochemistry 45(5). p.815-819
Abstract
The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in... (More)
The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in Nile perch triglycerides (TAG), while only 16% of this fatty acid was in sn-2 position in salmon oil TAG. (C) 2010 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DHA, EPA, Positional analysis, Enzymatic extraction, Salmon, Nile perch
in
Process Biochemistry
volume
45
issue
5
pages
815 - 819
publisher
Elsevier
external identifiers
  • wos:000277537000030
  • scopus:77950298900
ISSN
1873-3298
DOI
10.1016/j.procbio.2010.02.010
language
English
LU publication?
yes
id
514114e2-52da-4ada-93e0-ba32b1b7baf8 (old id 1617695)
date added to LUP
2010-06-21 13:21:09
date last changed
2018-05-29 11:11:36
@article{514114e2-52da-4ada-93e0-ba32b1b7baf8,
  abstract     = {The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in Nile perch triglycerides (TAG), while only 16% of this fatty acid was in sn-2 position in salmon oil TAG. (C) 2010 Elsevier Ltd. All rights reserved.},
  author       = {Mbatia, Betty and Adlercreutz, Dietlind and Adlercreutz, Patrick and Mahadhy, Ally and Mulaa, Francis and Mattiasson, Bo},
  issn         = {1873-3298},
  keyword      = {DHA,EPA,Positional analysis,Enzymatic extraction,Salmon,Nile perch},
  language     = {eng},
  number       = {5},
  pages        = {815--819},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads},
  url          = {http://dx.doi.org/10.1016/j.procbio.2010.02.010},
  volume       = {45},
  year         = {2010},
}