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Linear dsDNA Partitions Spontaneously into the Inverse Hexagonal Lyotropic Liquid Crystalline Phases of Phospholipids.

Black, Camilla F; Wilson, Richard J; Nylander, Tommy LU ; Dymond, Marcus K and Attard, George S (2010) In Journal of the American Chemical Society 132(28). p.9728-9732
Abstract
Recently, we reported that DNA associated with inverse hexagonal (H(II)) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase. (1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic H(II) phase throughout transcription. Here, we investigate the partitioning of DNA between an H(II) lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic... (More)
Recently, we reported that DNA associated with inverse hexagonal (H(II)) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase. (1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic H(II) phase throughout transcription. Here, we investigate the partitioning of DNA between an H(II) lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed H(II) liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the H(II) phase. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
volume
132
issue
28
pages
9728 - 9732
publisher
The American Chemical Society
external identifiers
  • wos:000280086800049
  • pmid:20575567
  • scopus:77954643179
ISSN
1520-5126
DOI
10.1021/ja101550c
language
English
LU publication?
yes
id
9ff50112-6802-4dc6-8eb1-a2f2fa29428a (old id 1625734)
date added to LUP
2010-08-23 12:53:02
date last changed
2018-05-29 10:59:00
@article{9ff50112-6802-4dc6-8eb1-a2f2fa29428a,
  abstract     = {Recently, we reported that DNA associated with inverse hexagonal (H(II)) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase. (1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic H(II) phase throughout transcription. Here, we investigate the partitioning of DNA between an H(II) lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed H(II) liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the H(II) phase.},
  author       = {Black, Camilla F and Wilson, Richard J and Nylander, Tommy and Dymond, Marcus K and Attard, George S},
  issn         = {1520-5126},
  language     = {eng},
  number       = {28},
  pages        = {9728--9732},
  publisher    = {The American Chemical Society},
  series       = {Journal of the American Chemical Society},
  title        = {Linear dsDNA Partitions Spontaneously into the Inverse Hexagonal Lyotropic Liquid Crystalline Phases of Phospholipids.},
  url          = {http://dx.doi.org/10.1021/ja101550c},
  volume       = {132},
  year         = {2010},
}