Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine.
(2010) In Nucleosides, Nucleotides & Nucleic Acids 29(4-6). p.445-448- Abstract
- Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1626122
- author
- Christoffersen, Stig LU ; Serra, Immacolata LU ; Terreni, M and Piskur, Jure LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nucleosides, Nucleotides & Nucleic Acids
- volume
- 29
- issue
- 4-6
- pages
- 445 - 448
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000278713200031
- scopus:77953692414
- pmid:20544534
- ISSN
- 1525-7770
- DOI
- 10.1080/15257771003741422
- language
- English
- LU publication?
- yes
- id
- a225d8a3-cfad-451d-9b8e-bdcedd43db1b (old id 1626122)
- date added to LUP
- 2016-04-01 10:26:21
- date last changed
- 2022-04-27 22:03:01
@article{a225d8a3-cfad-451d-9b8e-bdcedd43db1b, abstract = {{Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.}}, author = {{Christoffersen, Stig and Serra, Immacolata and Terreni, M and Piskur, Jure}}, issn = {{1525-7770}}, language = {{eng}}, number = {{4-6}}, pages = {{445--448}}, publisher = {{Taylor & Francis}}, series = {{Nucleosides, Nucleotides & Nucleic Acids}}, title = {{Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine.}}, url = {{http://dx.doi.org/10.1080/15257771003741422}}, doi = {{10.1080/15257771003741422}}, volume = {{29}}, year = {{2010}}, }