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Ribosylurea accumulates in yeast urc4 mutants.

Björnberg, Olof LU ; Vodnala, M; Domkin, V; Hofer, A; Rasmussen, Anna LU ; Andersen, G and Piskur, Jure LU (2010) In Nucleosides, Nucleotides & Nucleic Acids 29(4-6). p.433-437
Abstract
Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nucleosides, Nucleotides & Nucleic Acids
volume
29
issue
4-6
pages
433 - 437
publisher
Taylor & Francis
external identifiers
  • wos:000278713200029
  • scopus:77953701792
ISSN
1525-7770
DOI
10.1080/15257771003741265
language
English
LU publication?
yes
id
02c88c2a-bbe7-4314-b495-ed4b0424abaf (old id 1626129)
date added to LUP
2010-09-03 13:00:41
date last changed
2018-05-29 11:32:29
@article{02c88c2a-bbe7-4314-b495-ed4b0424abaf,
  abstract     = {Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.},
  author       = {Björnberg, Olof and Vodnala, M and Domkin, V and Hofer, A and Rasmussen, Anna and Andersen, G and Piskur, Jure},
  issn         = {1525-7770},
  language     = {eng},
  number       = {4-6},
  pages        = {433--437},
  publisher    = {Taylor & Francis},
  series       = {Nucleosides, Nucleotides & Nucleic Acids},
  title        = {Ribosylurea accumulates in yeast urc4 mutants.},
  url          = {http://dx.doi.org/10.1080/15257771003741265},
  volume       = {29},
  year         = {2010},
}