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Pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs.

Clausen, Anders Ranegaard LU ; Al Meani, S A L and Piskur, Jure LU (2010) In Nucleosides, Nucleotides & Nucleic Acids 29(4-6). p.359-362
Abstract
In the Pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (PmTK1), was identified. The PmTK1 gene was sub-cloned into Escherichia coli KY895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dFdC), 3'-azido-thymidine (AZT) and 5-fluoro-deoxyuridine (5F-dU). PmTK1 was over-expressed and purified with two different tags. Apparently, deoxyuridine (dU), and not thymidine (dT), is the preferred substrate. We suggest that PmTK1s could be employed as a species-specific activator of uracil-based nucleoside antibiotics.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nucleosides, Nucleotides & Nucleic Acids
volume
29
issue
4-6
pages
359 - 362
publisher
Taylor & Francis
external identifiers
  • wos:000278713200017
  • scopus:77953705611
ISSN
1525-7770
DOI
10.1080/15257771003729716
language
English
LU publication?
yes
id
d390c6bd-342e-443e-ab39-699ab05dbf6d (old id 1626139)
date added to LUP
2010-09-03 12:43:23
date last changed
2018-05-29 10:33:16
@article{d390c6bd-342e-443e-ab39-699ab05dbf6d,
  abstract     = {In the Pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (PmTK1), was identified. The PmTK1 gene was sub-cloned into Escherichia coli KY895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dFdC), 3'-azido-thymidine (AZT) and 5-fluoro-deoxyuridine (5F-dU). PmTK1 was over-expressed and purified with two different tags. Apparently, deoxyuridine (dU), and not thymidine (dT), is the preferred substrate. We suggest that PmTK1s could be employed as a species-specific activator of uracil-based nucleoside antibiotics.},
  author       = {Clausen, Anders Ranegaard and Al Meani, S A L and Piskur, Jure},
  issn         = {1525-7770},
  language     = {eng},
  number       = {4-6},
  pages        = {359--362},
  publisher    = {Taylor & Francis},
  series       = {Nucleosides, Nucleotides & Nucleic Acids},
  title        = {Pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs.},
  url          = {http://dx.doi.org/10.1080/15257771003729716},
  volume       = {29},
  year         = {2010},
}