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N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover.

Johansson, Jenny LU ; Voss, Ulrikke LU ; Kesavan, Gokul LU ; Kostetskii, Igor; Wierup, Nils LU ; Radice, Glenn L and Semb, Henrik LU (2010) In Genesis: The Journal of Genetics and Development2000-01-01+01:002011-01-01+01:00 48(6). p.374-381
Abstract
The cadherin family of cell adhesion molecules mediates adhesive interactions that are required for the formation and maintenance of tissues. Previously, we demonstrated that N-cadherin, which is required for numerous morphogenetic processes, is expressed in the pancreatic epithelium at E9.5, but later becomes restricted to endocrine aggregates in mice. To study the role of N-cadherin during pancreas formation and function we generated a tissue-specific knockout of N-cadherin in the early pancreatic epithelium by inter-crossing N-cadherin-floxed mice with Pdx1Cre mice. Analysis of pancreas-specific ablation of N-cadherin demonstrates that N-cadherin is dispensable for pancreatic development, but required for beta-cell granule turnover. The... (More)
The cadherin family of cell adhesion molecules mediates adhesive interactions that are required for the formation and maintenance of tissues. Previously, we demonstrated that N-cadherin, which is required for numerous morphogenetic processes, is expressed in the pancreatic epithelium at E9.5, but later becomes restricted to endocrine aggregates in mice. To study the role of N-cadherin during pancreas formation and function we generated a tissue-specific knockout of N-cadherin in the early pancreatic epithelium by inter-crossing N-cadherin-floxed mice with Pdx1Cre mice. Analysis of pancreas-specific ablation of N-cadherin demonstrates that N-cadherin is dispensable for pancreatic development, but required for beta-cell granule turnover. The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Genesis: The Journal of Genetics and Development2000-01-01+01:002011-01-01+01:00
volume
48
issue
6
pages
374 - 381
publisher
John Wiley & Sons
external identifiers
  • wos:000279030800004
  • pmid:20533404
  • scopus:77953510208
ISSN
1526-954X
DOI
10.1002/dvg.20628
language
English
LU publication?
yes
id
b8f533ad-1eb4-4837-a5f3-488737a1bfb0 (old id 1626249)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20533404?dopt=Abstract
date added to LUP
2010-07-05 09:55:10
date last changed
2018-05-29 11:00:47
@article{b8f533ad-1eb4-4837-a5f3-488737a1bfb0,
  abstract     = {The cadherin family of cell adhesion molecules mediates adhesive interactions that are required for the formation and maintenance of tissues. Previously, we demonstrated that N-cadherin, which is required for numerous morphogenetic processes, is expressed in the pancreatic epithelium at E9.5, but later becomes restricted to endocrine aggregates in mice. To study the role of N-cadherin during pancreas formation and function we generated a tissue-specific knockout of N-cadherin in the early pancreatic epithelium by inter-crossing N-cadherin-floxed mice with Pdx1Cre mice. Analysis of pancreas-specific ablation of N-cadherin demonstrates that N-cadherin is dispensable for pancreatic development, but required for beta-cell granule turnover. The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased.},
  author       = {Johansson, Jenny and Voss, Ulrikke and Kesavan, Gokul and Kostetskii, Igor and Wierup, Nils and Radice, Glenn L and Semb, Henrik},
  issn         = {1526-954X},
  language     = {eng},
  number       = {6},
  pages        = {374--381},
  publisher    = {John Wiley & Sons},
  series       = {Genesis: The Journal of Genetics and Development2000-01-01+01:002011-01-01+01:00},
  title        = {N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover.},
  url          = {http://dx.doi.org/10.1002/dvg.20628},
  volume       = {48},
  year         = {2010},
}