Advanced

Avoiding Acetate Accumulation in Escherichia coli Cultures Using Feedback Control of Glucose Feeding

Åkesson, Mats; Hagander, Per LU and Axelsson, Jan Peter (2001) In Biotechnology and Bioengineering 73(3). p.223-230
Abstract
An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally applicable as no strain specific information is needed andthe only sensor required is a standard dissolved oxygen probe. It alsogives the highest feed rate possible with respect to limitations fromoverflow metabolism and oxygen transfer, thus maximizing bioreactorproductivity.The strategy was implemented on three different laboratory-scale... (More)
An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally applicable as no strain specific information is needed andthe only sensor required is a standard dissolved oxygen probe. It alsogives the highest feed rate possible with respect to limitations fromoverflow metabolism and oxygen transfer, thus maximizing bioreactorproductivity.The strategy was implemented on three different laboratory-scale platformsand fed-batch cultivations under different operating conditions wereperformed with three recombinant strains, E. coli K-12 UL635,E. coli BL21(DE3), and E. coli K-12 UL634. In spite ofdisturbances from antifoam and induction of recombinant proteinproduction, the method reproducibly gave low concentrations of acetateand glucose.The ability to obtain favorable cultivation conditions independentlyof strain and operating conditions makes the presented strategy auseful tool, especially in situations where it is important to getgood results at the first attempt. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dissolved oxygen, process control, glucose, acetate, Escherichia coli, fed-batch cultivation
in
Biotechnology and Bioengineering
volume
73
issue
3
pages
223 - 230
publisher
John Wiley & Sons
external identifiers
  • scopus:0035810676
ISSN
1097-0290
DOI
10.1002/bit.1054
language
English
LU publication?
yes
id
a3d3ff52-3154-4c8c-b0e5-e451b1d5e67e (old id 162845)
date added to LUP
2007-07-11 11:09:28
date last changed
2018-07-15 03:35:43
@article{a3d3ff52-3154-4c8c-b0e5-e451b1d5e67e,
  abstract     = {An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally applicable as no strain specific information is needed andthe only sensor required is a standard dissolved oxygen probe. It alsogives the highest feed rate possible with respect to limitations fromoverflow metabolism and oxygen transfer, thus maximizing bioreactorproductivity.The strategy was implemented on three different laboratory-scale platformsand fed-batch cultivations under different operating conditions wereperformed with three recombinant strains, E. coli K-12 UL635,E. coli BL21(DE3), and E. coli K-12 UL634. In spite ofdisturbances from antifoam and induction of recombinant proteinproduction, the method reproducibly gave low concentrations of acetateand glucose.The ability to obtain favorable cultivation conditions independentlyof strain and operating conditions makes the presented strategy auseful tool, especially in situations where it is important to getgood results at the first attempt.},
  author       = {Åkesson, Mats and Hagander, Per and Axelsson, Jan Peter},
  issn         = {1097-0290},
  keyword      = {dissolved oxygen,process control,glucose,acetate,Escherichia coli,fed-batch cultivation},
  language     = {eng},
  number       = {3},
  pages        = {223--230},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Avoiding Acetate Accumulation in Escherichia coli Cultures Using Feedback Control of Glucose Feeding},
  url          = {http://dx.doi.org/10.1002/bit.1054},
  volume       = {73},
  year         = {2001},
}