Avoiding Acetate Accumulation in Escherichia coli Cultures Using Feedback Control of Glucose Feeding
(2001) In Biotechnology and Bioengineering 73(3). p.223-230- Abstract
- An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally applicable as no strain specific information is needed andthe only sensor required is a standard dissolved oxygen probe. It alsogives the highest feed rate possible with respect to limitations fromoverflow metabolism and oxygen transfer, thus maximizing bioreactorproductivity.The strategy was implemented on three different laboratory-scale... (More)
- An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally applicable as no strain specific information is needed andthe only sensor required is a standard dissolved oxygen probe. It alsogives the highest feed rate possible with respect to limitations fromoverflow metabolism and oxygen transfer, thus maximizing bioreactorproductivity.The strategy was implemented on three different laboratory-scale platformsand fed-batch cultivations under different operating conditions wereperformed with three recombinant strains, E. coli K-12 UL635,E. coli BL21(DE3), and E. coli K-12 UL634. In spite ofdisturbances from antifoam and induction of recombinant proteinproduction, the method reproducibly gave low concentrations of acetateand glucose.The ability to obtain favorable cultivation conditions independentlyof strain and operating conditions makes the presented strategy auseful tool, especially in situations where it is important to getgood results at the first attempt. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/162845
- author
- Åkesson, Mats ; Hagander, Per LU and Axelsson, Jan Peter
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- dissolved oxygen, process control, glucose, acetate, Escherichia coli, fed-batch cultivation
- in
- Biotechnology and Bioengineering
- volume
- 73
- issue
- 3
- pages
- 223 - 230
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0035810676
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.1054
- language
- English
- LU publication?
- yes
- id
- a3d3ff52-3154-4c8c-b0e5-e451b1d5e67e (old id 162845)
- date added to LUP
- 2016-04-01 12:17:21
- date last changed
- 2025-04-04 14:05:58
@article{a3d3ff52-3154-4c8c-b0e5-e451b1d5e67e,
abstract = {{An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally applicable as no strain specific information is needed andthe only sensor required is a standard dissolved oxygen probe. It alsogives the highest feed rate possible with respect to limitations fromoverflow metabolism and oxygen transfer, thus maximizing bioreactorproductivity.The strategy was implemented on three different laboratory-scale platformsand fed-batch cultivations under different operating conditions wereperformed with three recombinant strains, E. coli K-12 UL635,E. coli BL21(DE3), and E. coli K-12 UL634. In spite ofdisturbances from antifoam and induction of recombinant proteinproduction, the method reproducibly gave low concentrations of acetateand glucose.The ability to obtain favorable cultivation conditions independentlyof strain and operating conditions makes the presented strategy auseful tool, especially in situations where it is important to getgood results at the first attempt.}},
author = {{Åkesson, Mats and Hagander, Per and Axelsson, Jan Peter}},
issn = {{1097-0290}},
keywords = {{dissolved oxygen; process control; glucose; acetate; Escherichia coli; fed-batch cultivation}},
language = {{eng}},
number = {{3}},
pages = {{223--230}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Avoiding Acetate Accumulation in Escherichia coli Cultures Using Feedback Control of Glucose Feeding}},
url = {{http://dx.doi.org/10.1002/bit.1054}},
doi = {{10.1002/bit.1054}},
volume = {{73}},
year = {{2001}},
}