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Probing Control of Fed-batch Cultures: Analysis and Tuning

Åkesson, Mats; Hagander, Per LU and Axelsson, Jan Peter (2001) In Control Engineering Practice 9(7). p.709-723
Abstract
Production of various proteins can today be made using genetically modified Escherichia coli bacteria. In cultivations of E. coli it is important to avoid accumulation of the by-product acetate. Formation of acetate occurs when the specific glucose uptake exceeds a critical value and can be avoided by a proper feeding strategy. A difficulty is that the critical glucose uptake often is poorly known and even time varying. We here analyze an approach for control of glucose feeding that enables feeding at the critical glucose uptake without prior information. The key idea is to superimpose a probing signal to the feed rate in order to obtain information used to determine if the feed rate should be increased or decreased. The main contribution... (More)
Production of various proteins can today be made using genetically modified Escherichia coli bacteria. In cultivations of E. coli it is important to avoid accumulation of the by-product acetate. Formation of acetate occurs when the specific glucose uptake exceeds a critical value and can be avoided by a proper feeding strategy. A difficulty is that the critical glucose uptake often is poorly known and even time varying. We here analyze an approach for control of glucose feeding that enables feeding at the critical glucose uptake without prior information. The key idea is to superimpose a probing signal to the feed rate in order to obtain information used to determine if the feed rate should be increased or decreased. The main contribution of this paper is to derive guidelines for tuning of the probing controller. A sufficient condition for stability is presented. By introducing proportional and proportional¯integral control action it is possible to improve performance with an unchanged stability guarantee. This also gives a possibility to maintain a minimum specified distance to the critical glucose uptake where acetate formation starts. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Control system analysis, Fermentation processes, Process control, Biotechnology, Probing
in
Control Engineering Practice
volume
9
issue
7
pages
709 - 723
publisher
Elsevier
external identifiers
  • scopus:0035392147
ISSN
0967-0661
DOI
10.1016/S0967-0661(01)00020-X
language
English
LU publication?
yes
id
d8f3a723-adfb-4672-9096-63e84bcd1940 (old id 162850)
date added to LUP
2007-07-04 14:38:17
date last changed
2018-01-07 06:04:33
@article{d8f3a723-adfb-4672-9096-63e84bcd1940,
  abstract     = {Production of various proteins can today be made using genetically modified Escherichia coli bacteria. In cultivations of E. coli it is important to avoid accumulation of the by-product acetate. Formation of acetate occurs when the specific glucose uptake exceeds a critical value and can be avoided by a proper feeding strategy. A difficulty is that the critical glucose uptake often is poorly known and even time varying. We here analyze an approach for control of glucose feeding that enables feeding at the critical glucose uptake without prior information. The key idea is to superimpose a probing signal to the feed rate in order to obtain information used to determine if the feed rate should be increased or decreased. The main contribution of this paper is to derive guidelines for tuning of the probing controller. A sufficient condition for stability is presented. By introducing proportional and proportional¯integral control action it is possible to improve performance with an unchanged stability guarantee. This also gives a possibility to maintain a minimum specified distance to the critical glucose uptake where acetate formation starts.},
  author       = {Åkesson, Mats and Hagander, Per and Axelsson, Jan Peter},
  issn         = {0967-0661},
  keyword      = {Control system analysis,Fermentation processes,Process control,Biotechnology,Probing},
  language     = {eng},
  number       = {7},
  pages        = {709--723},
  publisher    = {Elsevier},
  series       = {Control Engineering Practice},
  title        = {Probing Control of Fed-batch Cultures: Analysis and Tuning},
  url          = {http://dx.doi.org/10.1016/S0967-0661(01)00020-X},
  volume       = {9},
  year         = {2001},
}