Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose
(2010) In Biosensors & Bioelectronics 25(10). p.2205-2211- Abstract
- In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the... (More)
- In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration. (C) 2010 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1631580
- author
- Asif, Muhammad H. ; Ali, Syed M. Usman ; Nur, Omer ; Willander, Magnus ; Brannmark, Cecilia ; Stralfors, Peter ; Englund, Ulrika H. ; Elinder, Fredrik and Danielsson, Bengt LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Intracellular glucose, ZnO nanorods, Functionalisation, sensor, Electrochemical
- in
- Biosensors & Bioelectronics
- volume
- 25
- issue
- 10
- pages
- 2205 - 2211
- publisher
- Elsevier
- external identifiers
-
- wos:000278702600004
- scopus:77952567384
- pmid:20303253
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2010.02.025
- language
- English
- LU publication?
- yes
- id
- 6909007a-d399-4578-9c55-dc19df88a99d (old id 1631580)
- date added to LUP
- 2016-04-01 13:14:05
- date last changed
- 2025-01-17 08:21:04
@article{6909007a-d399-4578-9c55-dc19df88a99d, abstract = {{In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration. (C) 2010 Elsevier B.V. All rights reserved.}}, author = {{Asif, Muhammad H. and Ali, Syed M. Usman and Nur, Omer and Willander, Magnus and Brannmark, Cecilia and Stralfors, Peter and Englund, Ulrika H. and Elinder, Fredrik and Danielsson, Bengt}}, issn = {{1873-4235}}, keywords = {{Intracellular glucose; ZnO nanorods; Functionalisation; sensor; Electrochemical}}, language = {{eng}}, number = {{10}}, pages = {{2205--2211}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose}}, url = {{http://dx.doi.org/10.1016/j.bios.2010.02.025}}, doi = {{10.1016/j.bios.2010.02.025}}, volume = {{25}}, year = {{2010}}, }