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Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose

Asif, Muhammad H.; Ali, Syed M. Usman; Nur, Omer; Willander, Magnus; Brannmark, Cecilia; Stralfors, Peter; Englund, Ulrika H.; Elinder, Fredrik and Danielsson, Bengt LU (2010) In Biosensors & Bioelectronics 25(10). p.2205-2211
Abstract
In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the... (More)
In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration. (C) 2010 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Intracellular glucose, ZnO nanorods, Functionalisation, sensor, Electrochemical
in
Biosensors & Bioelectronics
volume
25
issue
10
pages
2205 - 2211
publisher
Elsevier
external identifiers
  • wos:000278702600004
  • scopus:77952567384
ISSN
1873-4235
DOI
10.1016/j.bios.2010.02.025
language
English
LU publication?
yes
id
6909007a-d399-4578-9c55-dc19df88a99d (old id 1631580)
date added to LUP
2010-07-22 14:00:42
date last changed
2018-07-15 03:44:13
@article{6909007a-d399-4578-9c55-dc19df88a99d,
  abstract     = {In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration. (C) 2010 Elsevier B.V. All rights reserved.},
  author       = {Asif, Muhammad H. and Ali, Syed M. Usman and Nur, Omer and Willander, Magnus and Brannmark, Cecilia and Stralfors, Peter and Englund, Ulrika H. and Elinder, Fredrik and Danielsson, Bengt},
  issn         = {1873-4235},
  keyword      = {Intracellular glucose,ZnO nanorods,Functionalisation,sensor,Electrochemical},
  language     = {eng},
  number       = {10},
  pages        = {2205--2211},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose},
  url          = {http://dx.doi.org/10.1016/j.bios.2010.02.025},
  volume       = {25},
  year         = {2010},
}