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Retinal progenitor cell xenografts to the pig retina: immunological reactions.

Warfvinge, Karin LU orcid ; Kiilgaard, Jens F ; Klassen, Henry ; Zamiri, Parisa ; Scherfig, Erik ; Streilein, Wayne ; Prause, Jan U and Young, Michael J (2006) In Cell Transplantation 15(7). p.603-612
Abstract
We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-cosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2-5 weeks. Serum from naive and... (More)
We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-cosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2-5 weeks. Serum from naive and RPC-xenografted pigs contained significant levels of preformed IgG and IgM antibodies against murine antigens. Xenogeneic RPCs transplanted to the porcine SRS induced mononuclear infiltration in the choroid with graft rejection occurring over 2-5 weeks. Serum analysis confirmed that mice and pigs are discordant species; however, a cell-mediated acute mechanism appears to be responsible, rather than an antibody-mediated rejection. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
stem cell, xenografts, transplant, retina
in
Cell Transplantation
volume
15
issue
7
pages
603 - 612
publisher
Cognizant Communication Corporation
external identifiers
  • wos:000242553500004
  • scopus:33845418755
ISSN
1555-3892
language
English
LU publication?
yes
id
165258a1-beb3-46fd-9867-1f3a5f9edcce (old id 163930)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17176612&dopt=Abstract
date added to LUP
2016-04-01 11:53:23
date last changed
2021-08-04 04:15:34
@article{165258a1-beb3-46fd-9867-1f3a5f9edcce,
  abstract     = {We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-cosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2-5 weeks. Serum from naive and RPC-xenografted pigs contained significant levels of preformed IgG and IgM antibodies against murine antigens. Xenogeneic RPCs transplanted to the porcine SRS induced mononuclear infiltration in the choroid with graft rejection occurring over 2-5 weeks. Serum analysis confirmed that mice and pigs are discordant species; however, a cell-mediated acute mechanism appears to be responsible, rather than an antibody-mediated rejection.},
  author       = {Warfvinge, Karin and Kiilgaard, Jens F and Klassen, Henry and Zamiri, Parisa and Scherfig, Erik and Streilein, Wayne and Prause, Jan U and Young, Michael J},
  issn         = {1555-3892},
  language     = {eng},
  number       = {7},
  pages        = {603--612},
  publisher    = {Cognizant Communication Corporation},
  series       = {Cell Transplantation},
  title        = {Retinal progenitor cell xenografts to the pig retina: immunological reactions.},
  url          = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17176612&dopt=Abstract},
  volume       = {15},
  year         = {2006},
}