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Antifungal activity of C3a and C3a-derived peptides against Candida.

Sonesson, Andreas LU ; Ringstad, Lovisa; Nordahl, Emma LU ; Malmsten, Martin; Mörgelin, Matthias LU and Schmidtchen, Artur LU (2007) In Biochimica et Biophysica Acta - Biomembranes 1768(2). p.346-353
Abstract
Antimicrobial peptides are generated during activation of the complement system [Nordahl et al. Proc. Nat1. Acad. Sci. U. S. A. 2004, 101:16879-16884]. Here we show that the anaphylatoxin C3a exerts antimicrobial effects against the yeast Candida. Fluorescence microscopy and electron microscopy analysis demonstrated that C3a-derived peptides bound to the cell surface of Candida, and induced membrane perturbations and release of extracellular material. Various Candida isolates were found to induce complement degradation, leading to generation of C3a. Arginine residues were found to be critical for the antifungal and membrane breaking activity of a C3a-derived antimicrobial peptide, CNY21 (C3a; Cys(57)-Arg(77)). A CNY21 variant with... (More)
Antimicrobial peptides are generated during activation of the complement system [Nordahl et al. Proc. Nat1. Acad. Sci. U. S. A. 2004, 101:16879-16884]. Here we show that the anaphylatoxin C3a exerts antimicrobial effects against the yeast Candida. Fluorescence microscopy and electron microscopy analysis demonstrated that C3a-derived peptides bound to the cell surface of Candida, and induced membrane perturbations and release of extracellular material. Various Candida isolates were found to induce complement degradation, leading to generation of C3a. Arginine residues were found to be critical for the antifungal and membrane breaking activity of a C3a-derived antimicrobial peptide, CNY21 (C3a; Cys(57)-Arg(77)). A CNY21 variant with increased positive net charge displayed enhanced antifungal activity. Thus, C3a-derived peptides can be utilized as templates in the development of peptide-based antifungal therapies. (c) 2006 Elsevier B.V All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
atopic dermatitis, Candida, innate immunity, antimicrobial peptides, complement, structure-activity relationship study
in
Biochimica et Biophysica Acta - Biomembranes
volume
1768
issue
2
pages
346 - 353
publisher
Elsevier
external identifiers
  • wos:000244384600017
  • scopus:33846378792
ISSN
0005-2736
DOI
10.1016/j.bbamem.2006.10.017
language
English
LU publication?
yes
id
ebdd70c8-6c3c-4515-93e6-1452aecbadf6 (old id 163998)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17169328&dopt=Abstract
date added to LUP
2007-07-12 15:30:19
date last changed
2017-10-01 04:38:26
@article{ebdd70c8-6c3c-4515-93e6-1452aecbadf6,
  abstract     = {Antimicrobial peptides are generated during activation of the complement system [Nordahl et al. Proc. Nat1. Acad. Sci. U. S. A. 2004, 101:16879-16884]. Here we show that the anaphylatoxin C3a exerts antimicrobial effects against the yeast Candida. Fluorescence microscopy and electron microscopy analysis demonstrated that C3a-derived peptides bound to the cell surface of Candida, and induced membrane perturbations and release of extracellular material. Various Candida isolates were found to induce complement degradation, leading to generation of C3a. Arginine residues were found to be critical for the antifungal and membrane breaking activity of a C3a-derived antimicrobial peptide, CNY21 (C3a; Cys(57)-Arg(77)). A CNY21 variant with increased positive net charge displayed enhanced antifungal activity. Thus, C3a-derived peptides can be utilized as templates in the development of peptide-based antifungal therapies. (c) 2006 Elsevier B.V All rights reserved.},
  author       = {Sonesson, Andreas and Ringstad, Lovisa and Nordahl, Emma and Malmsten, Martin and Mörgelin, Matthias and Schmidtchen, Artur},
  issn         = {0005-2736},
  keyword      = {atopic dermatitis,Candida,innate immunity,antimicrobial peptides,complement,structure-activity relationship study},
  language     = {eng},
  number       = {2},
  pages        = {346--353},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Biomembranes},
  title        = {Antifungal activity of C3a and C3a-derived peptides against Candida.},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2006.10.017},
  volume       = {1768},
  year         = {2007},
}