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Non-contact acoustic cell trapping in disposable glass capillaries.

Hammarström, Björn LU ; Evander, Mikael LU ; Barbeau, Herve; Bruzelius, Mattias; Larsson, Jörgen; Laurell, Thomas LU and Nilsson, Johan LU (2010) In Lab on A Chip 10. p.2251-2257
Abstract
Non-contact trapping using acoustic standing waves has shown promising results in cell-based research lately. However, the devices demonstrated are normally fabricated using microfabrication or precision machining methods leading to a high unit cost. In e.g. clinical or forensic applications avoiding cross-contamination, carryover or infection is of outmost importance. In these applications disposable devices are key elements, thus making the cost per unit a critical factor. A solution is presented here where low-cost off-the-shelf glass capillaries are used as resonators for standing wave trapping. Single-mode as well as multi-node trapping is demonstrated with an excellent agreement between simulated and experimentally found operation... (More)
Non-contact trapping using acoustic standing waves has shown promising results in cell-based research lately. However, the devices demonstrated are normally fabricated using microfabrication or precision machining methods leading to a high unit cost. In e.g. clinical or forensic applications avoiding cross-contamination, carryover or infection is of outmost importance. In these applications disposable devices are key elements, thus making the cost per unit a critical factor. A solution is presented here where low-cost off-the-shelf glass capillaries are used as resonators for standing wave trapping. Single-mode as well as multi-node trapping is demonstrated with an excellent agreement between simulated and experimentally found operation frequencies. Single particle trapping is verified at 7.53 MHz with a trapping force on a 10 mum particle of up to 1.27 nN. The non-contact trapping is proved using confocal microscopy. Finally, an application is presented where the capillary is used as a pipette for aspirating, trapping and dispensing red blood cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
10
pages
2251 - 2257
publisher
Royal Society of Chemistry
external identifiers
  • pmid:20589284
  • wos:000280812600011
  • scopus:77955537975
ISSN
1473-0189
DOI
10.1039/c004504g
language
English
LU publication?
yes
id
9289d1a4-48ad-4f92-92cc-97902d4ad137 (old id 1645462)
date added to LUP
2010-08-10 14:33:19
date last changed
2018-07-15 03:07:20
@article{9289d1a4-48ad-4f92-92cc-97902d4ad137,
  abstract     = {Non-contact trapping using acoustic standing waves has shown promising results in cell-based research lately. However, the devices demonstrated are normally fabricated using microfabrication or precision machining methods leading to a high unit cost. In e.g. clinical or forensic applications avoiding cross-contamination, carryover or infection is of outmost importance. In these applications disposable devices are key elements, thus making the cost per unit a critical factor. A solution is presented here where low-cost off-the-shelf glass capillaries are used as resonators for standing wave trapping. Single-mode as well as multi-node trapping is demonstrated with an excellent agreement between simulated and experimentally found operation frequencies. Single particle trapping is verified at 7.53 MHz with a trapping force on a 10 mum particle of up to 1.27 nN. The non-contact trapping is proved using confocal microscopy. Finally, an application is presented where the capillary is used as a pipette for aspirating, trapping and dispensing red blood cells.},
  author       = {Hammarström, Björn and Evander, Mikael and Barbeau, Herve and Bruzelius, Mattias and Larsson, Jörgen and Laurell, Thomas and Nilsson, Johan},
  issn         = {1473-0189},
  language     = {eng},
  pages        = {2251--2257},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Non-contact acoustic cell trapping in disposable glass capillaries.},
  url          = {http://dx.doi.org/10.1039/c004504g},
  volume       = {10},
  year         = {2010},
}