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Altered peripheral amino acid profile indicate a systemic impact of active celiac disease and a possible role of amino acids in disease pathogenesis

Naluai, Åsa Torinsson; Vafa, Ladan Saadat; Gudjonsdottir, Audur H.; Arnell, Henrik; Browaldh, Lars; Nilsson, Staffan and Agardh, Daniel LU (2018) In PLoS ONE 13(3).
Abstract

Background: We have previously performed a Genome Wide Association and linkage study that indicated a new disease triggering mechanism involving amino acid metabolism and nutrient sensing signaling pathways. Objective: The aim of this study was to investigate if plasma amino acid levels differed among children with celiac disease compared with disease controls. Materials and methods: Fasting plasma samples from 141 children with celiac disease and 129 non-celiac disease controls, were analyzed for amino acid levels by liquid chromatography-tandem mass spectrometry (LC/MS). A general linear model using age and experimental effects as covariates was used to compare amino acid levels between children with a diagnosis of celiac disease and... (More)

Background: We have previously performed a Genome Wide Association and linkage study that indicated a new disease triggering mechanism involving amino acid metabolism and nutrient sensing signaling pathways. Objective: The aim of this study was to investigate if plasma amino acid levels differed among children with celiac disease compared with disease controls. Materials and methods: Fasting plasma samples from 141 children with celiac disease and 129 non-celiac disease controls, were analyzed for amino acid levels by liquid chromatography-tandem mass spectrometry (LC/MS). A general linear model using age and experimental effects as covariates was used to compare amino acid levels between children with a diagnosis of celiac disease and controls. Results: Seven out of twenty-three analyzed amino acids were elevated in children with celiac disease compared with controls (tryptophan, taurine, glutamic acid, proline, ornithine, alanine and methionine). The significance of the individual amino acids do not survive multiple correction, however, multivariate analyses of the amino acid profile showed significantly altered amino acid levels in children with celiac disease overall and after correction for age, sex and experimental effects (p = 8.4 × 10-8). Conclusion: These findings support the idea that amino acids could influence systemic inflammation and play a possible role in disease pathogenesis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
13
issue
3
publisher
Public Library of Science
external identifiers
  • scopus:85043780412
ISSN
1932-6203
DOI
10.1371/journal.pone.0193764
language
English
LU publication?
yes
id
16479299-be42-4cb7-9515-434e2f8a84cb
date added to LUP
2018-03-27 13:46:52
date last changed
2019-02-20 11:12:06
@article{16479299-be42-4cb7-9515-434e2f8a84cb,
  abstract     = {<p>Background: We have previously performed a Genome Wide Association and linkage study that indicated a new disease triggering mechanism involving amino acid metabolism and nutrient sensing signaling pathways. Objective: The aim of this study was to investigate if plasma amino acid levels differed among children with celiac disease compared with disease controls. Materials and methods: Fasting plasma samples from 141 children with celiac disease and 129 non-celiac disease controls, were analyzed for amino acid levels by liquid chromatography-tandem mass spectrometry (LC/MS). A general linear model using age and experimental effects as covariates was used to compare amino acid levels between children with a diagnosis of celiac disease and controls. Results: Seven out of twenty-three analyzed amino acids were elevated in children with celiac disease compared with controls (tryptophan, taurine, glutamic acid, proline, ornithine, alanine and methionine). The significance of the individual amino acids do not survive multiple correction, however, multivariate analyses of the amino acid profile showed significantly altered amino acid levels in children with celiac disease overall and after correction for age, sex and experimental effects (p = 8.4 × 10<sup>-8</sup>). Conclusion: These findings support the idea that amino acids could influence systemic inflammation and play a possible role in disease pathogenesis.</p>},
  articleno    = {e0193764},
  author       = {Naluai, Åsa Torinsson and Vafa, Ladan Saadat and Gudjonsdottir, Audur H. and Arnell, Henrik and Browaldh, Lars and Nilsson, Staffan and Agardh, Daniel},
  issn         = {1932-6203},
  language     = {eng},
  month        = {03},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Altered peripheral amino acid profile indicate a systemic impact of active celiac disease and a possible role of amino acids in disease pathogenesis},
  url          = {http://dx.doi.org/10.1371/journal.pone.0193764},
  volume       = {13},
  year         = {2018},
}