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Deletion of a major neutralizing epitope of human papillomavirus type 16 virus-like particles.

Ryding, Janka LU ; Dahlberg, L; Wallen-Ohman, M and Dillner, Joakim LU (2007) In Journal of General Virology 88(Pt 3). p.792-802
Abstract
Human papillomavirus type 16 (HPV-16) is a major cause of human cancer. Effective prophylactic vaccines are based on type-specific neutralizing antibodies. A major neutralizing epitope has been defined by the monoclonal antibody H16.V5. To investigate the importance of this epitope for overall immunogenicity of HPV-16, HPV-16 virus-like particles devoid of the H16.V5 epitope were engineered by site-directed mutagenesis of ten non-conserved, surface-exposed residues. Removal of the H16.V5-defined epitope had only a marginal effect on antigenic reactivity with antibodies in sera from infected subjects, but affected immunogenicity in experimental immunization of mice, with reduced induction of both antibody responses and CTL responses.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
88
issue
Pt 3
pages
792 - 802
publisher
Society for General Microbiology
external identifiers
  • wos:000245045500008
  • scopus:33847625099
ISSN
1465-2099
DOI
10.1099/vir.0.82449-0
language
English
LU publication?
yes
id
e82eea76-416b-4885-8118-6075e522ceda (old id 165405)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17325351&dopt=Abstract
date added to LUP
2007-07-26 16:11:20
date last changed
2017-03-12 04:08:00
@article{e82eea76-416b-4885-8118-6075e522ceda,
  abstract     = {Human papillomavirus type 16 (HPV-16) is a major cause of human cancer. Effective prophylactic vaccines are based on type-specific neutralizing antibodies. A major neutralizing epitope has been defined by the monoclonal antibody H16.V5. To investigate the importance of this epitope for overall immunogenicity of HPV-16, HPV-16 virus-like particles devoid of the H16.V5 epitope were engineered by site-directed mutagenesis of ten non-conserved, surface-exposed residues. Removal of the H16.V5-defined epitope had only a marginal effect on antigenic reactivity with antibodies in sera from infected subjects, but affected immunogenicity in experimental immunization of mice, with reduced induction of both antibody responses and CTL responses.},
  author       = {Ryding, Janka and Dahlberg, L and Wallen-Ohman, M and Dillner, Joakim},
  issn         = {1465-2099},
  language     = {eng},
  number       = {Pt 3},
  pages        = {792--802},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {Deletion of a major neutralizing epitope of human papillomavirus type 16 virus-like particles.},
  url          = {http://dx.doi.org/10.1099/vir.0.82449-0},
  volume       = {88},
  year         = {2007},
}