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Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B.

Lindh, Rebecka LU ; Ahmad, Faiyaz; Resjö, Svante LU ; James, Peter LU ; Yang, Jeong S; Fales, Henry M; Manganiello, Vincent and Degerman, Eva LU (2007) In Biochimica et Biophysica Acta: Molecular Cell Research 1773(4). p.584-592
Abstract
Phosphodiesterase 3B (PDE3B) is an important component of insulin and cAMP-dependent signalling pathways. In order to study phosphorylation of PDE3B, we have used an adenoviral system to express recombinant flag-tagged PDE3B in primary rat adipocytes and H4IIE hepatoma cells. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. We found that PDE3B is multisite phosphorylated in adipocytes and H4IIE hepatoma cells in response to all these stimuli. Several sites were identified; serine (S)273, S296, S421, S424/5, S474 and S536 were phosphorylated in adipocyte as well as H4IIE... (More)
Phosphodiesterase 3B (PDE3B) is an important component of insulin and cAMP-dependent signalling pathways. In order to study phosphorylation of PDE3B, we have used an adenoviral system to express recombinant flag-tagged PDE3B in primary rat adipocytes and H4IIE hepatoma cells. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. We found that PDE3B is multisite phosphorylated in adipocytes and H4IIE hepatoma cells in response to all these stimuli. Several sites were identified; serine (S)273, S296, S421, S424/5, S474 and S536 were phosphorylated in adipocyte as well as H4IIE hepatoma cells whereas S277 and S507 were phosphorylated in hepatoma cells only. Several of the sites were phosphorylated by insulin as well as cAMP-increasing hormones indicating integration of the two signalling pathways upstream of PDE3B, maybe at the level of protein kinase B. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phosphorylation, PDE3B, Adipocyte, Insulin, Hepatocyte, cAMP
in
Biochimica et Biophysica Acta: Molecular Cell Research
volume
1773
issue
4
pages
584 - 592
publisher
Elsevier
external identifiers
  • wos:000245784700013
  • scopus:33947326436
ISSN
0167-4889
DOI
10.1016/j.bbamcr.2007.01.010
language
English
LU publication?
yes
id
a8ffccb4-49e0-4b59-ac92-7825027a1884 (old id 165445)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17320989&dopt=Abstract
date added to LUP
2007-07-16 16:13:28
date last changed
2017-01-01 07:00:09
@article{a8ffccb4-49e0-4b59-ac92-7825027a1884,
  abstract     = {Phosphodiesterase 3B (PDE3B) is an important component of insulin and cAMP-dependent signalling pathways. In order to study phosphorylation of PDE3B, we have used an adenoviral system to express recombinant flag-tagged PDE3B in primary rat adipocytes and H4IIE hepatoma cells. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. We found that PDE3B is multisite phosphorylated in adipocytes and H4IIE hepatoma cells in response to all these stimuli. Several sites were identified; serine (S)273, S296, S421, S424/5, S474 and S536 were phosphorylated in adipocyte as well as H4IIE hepatoma cells whereas S277 and S507 were phosphorylated in hepatoma cells only. Several of the sites were phosphorylated by insulin as well as cAMP-increasing hormones indicating integration of the two signalling pathways upstream of PDE3B, maybe at the level of protein kinase B.},
  author       = {Lindh, Rebecka and Ahmad, Faiyaz and Resjö, Svante and James, Peter and Yang, Jeong S and Fales, Henry M and Manganiello, Vincent and Degerman, Eva},
  issn         = {0167-4889},
  keyword      = {Phosphorylation,PDE3B,Adipocyte,Insulin,Hepatocyte,cAMP},
  language     = {eng},
  number       = {4},
  pages        = {584--592},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta: Molecular Cell Research},
  title        = {Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B.},
  url          = {http://dx.doi.org/10.1016/j.bbamcr.2007.01.010},
  volume       = {1773},
  year         = {2007},
}