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Gene expression demonstrates increased resilience toward harmful inflammatory stimuli in the proliferating epidermis of human skin wounds

Roupé, Markus LU ; Alberius, Per LU ; Schmidtchen, Artur LU and Sørensen, Ole E LU (2010) In Experimental Dermatology 19(8). p.329-332
Abstract
We examined the epidermal gene expression during the proliferative phase of wound healing. Matrix metalloproteases were the group of proteases most prominently up-regulated in skin wounds, whereas serine protease inhibitors were the most strongly up-regulated protease inhibitors. Furthermore, we found down-regulation of genes involved in the extrinsic pathway of apoptosis. This together with the up-regulation of inhibitors of leukocyte serine proteases likely represents a protective step to ensure survival of keratinocytes in the inflammatory wound environment. The down-regulation of proapoptotic genes in the extrinsic pathway of apoptosis was not accompanied by a down-regulation of receptors indicating that the keratinocytes in skin... (More)
We examined the epidermal gene expression during the proliferative phase of wound healing. Matrix metalloproteases were the group of proteases most prominently up-regulated in skin wounds, whereas serine protease inhibitors were the most strongly up-regulated protease inhibitors. Furthermore, we found down-regulation of genes involved in the extrinsic pathway of apoptosis. This together with the up-regulation of inhibitors of leukocyte serine proteases likely represents a protective step to ensure survival of keratinocytes in the inflammatory wound environment. The down-regulation of proapoptotic genes in the extrinsic pathway of apoptosis was not accompanied by a down-regulation of receptors indicating that the keratinocytes in skin wounds did not become less responsive to external stimuli. Examining the transcription factor binding sites in the promoters of the most differentially expressed genes between normal skin and skin wounds a significant overrepresentation of binding sites were found for STAT-5, SRY and members of the FOXO-family of transcription factors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transcription factors, inflammation, Apoptosis, gene expression, wound, healing
in
Experimental Dermatology
volume
19
issue
8
pages
329 - 332
publisher
Wiley-Blackwell
external identifiers
  • wos:000280146200053
  • scopus:77954856727
ISSN
0906-6705
DOI
10.1111/j.1600-0625.2009.01038.x
language
English
LU publication?
yes
id
6849140c-53fd-4b24-9199-8ec0f14c8029 (old id 1654970)
date added to LUP
2010-08-30 13:52:09
date last changed
2018-05-29 10:21:10
@article{6849140c-53fd-4b24-9199-8ec0f14c8029,
  abstract     = {We examined the epidermal gene expression during the proliferative phase of wound healing. Matrix metalloproteases were the group of proteases most prominently up-regulated in skin wounds, whereas serine protease inhibitors were the most strongly up-regulated protease inhibitors. Furthermore, we found down-regulation of genes involved in the extrinsic pathway of apoptosis. This together with the up-regulation of inhibitors of leukocyte serine proteases likely represents a protective step to ensure survival of keratinocytes in the inflammatory wound environment. The down-regulation of proapoptotic genes in the extrinsic pathway of apoptosis was not accompanied by a down-regulation of receptors indicating that the keratinocytes in skin wounds did not become less responsive to external stimuli. Examining the transcription factor binding sites in the promoters of the most differentially expressed genes between normal skin and skin wounds a significant overrepresentation of binding sites were found for STAT-5, SRY and members of the FOXO-family of transcription factors.},
  author       = {Roupé, Markus and Alberius, Per and Schmidtchen, Artur and Sørensen, Ole E},
  issn         = {0906-6705},
  keyword      = {transcription factors,inflammation,Apoptosis,gene expression,wound,healing},
  language     = {eng},
  number       = {8},
  pages        = {329--332},
  publisher    = {Wiley-Blackwell},
  series       = {Experimental Dermatology},
  title        = {Gene expression demonstrates increased resilience toward harmful inflammatory stimuli in the proliferating epidermis of human skin wounds},
  url          = {http://dx.doi.org/10.1111/j.1600-0625.2009.01038.x},
  volume       = {19},
  year         = {2010},
}