Advanced

Effect of a Novel Botanical Agent Drynol Cibotin on Human Osteoblast Cells and Implications for Osteoporosis: Promotion of Cell Growth, Calcium Uptake and Collagen Production

Wegiel, Barbara and Persson, Jenny L LU (2010) In Phytotherapy Research 24. p.139-147
Abstract
Osteoporosis is a widespread problem afflicting millions of people. Drynol Cibotinis is a newly developed proprietary botanical combination of eight botanicals including Angelica sinensis, Glycine max, Wild yam, Ligustrum lucidum, Astragalus membranaceus, Cuscuta chinensis, Psoraleae corylifoliae, and Drynaria fortune. Each of the botanicals has been used in traditional Chinese medicine to treat osteoporosis. The effect of Drynol Cibotinis, with the specific combination of these anti-osteoporosis botanicals for promoting bone growth, was examined in this study. The effects of Drynol Cibotin on cell growth, apoptosis, cell spreading, calcium uptake and production of bone matrix proteins Collagen I and Laminin B2 on human osteoblast cells... (More)
Osteoporosis is a widespread problem afflicting millions of people. Drynol Cibotinis is a newly developed proprietary botanical combination of eight botanicals including Angelica sinensis, Glycine max, Wild yam, Ligustrum lucidum, Astragalus membranaceus, Cuscuta chinensis, Psoraleae corylifoliae, and Drynaria fortune. Each of the botanicals has been used in traditional Chinese medicine to treat osteoporosis. The effect of Drynol Cibotinis, with the specific combination of these anti-osteoporosis botanicals for promoting bone growth, was examined in this study. The effects of Drynol Cibotin on cell growth, apoptosis, cell spreading, calcium uptake and production of bone matrix proteins Collagen I and Laminin B2 on human osteoblast cells were assessed by BrdU incorporation, TUNEL assay, cell staining, intracellular Ca2+ measurement and Western blot analysis. The results showed that Drynol Cibotin significantly increased cell proliferation and inhibited apoptosis in osteoblasts (P < 0.01). In addition, Drynol Cibotin was found to promote cell spreading and greatly increase calcium uptake both instantaneously and in the long term (P < 0.01). Furthermore, Drynol Cibotin significantly increased production of two key extracellular matrix proteins in bone cells: Collagen I and Laminin B2. These results indicate that Drynol Cibotin alone or in combination with amino acids and vitamins may have prophylactic potentials in osteoporosis. Copyright (C) 2009 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Drynol Cibotin, collagen, calcium, bone, osteoporosis
in
Phytotherapy Research
volume
24
pages
139 - 147
publisher
John Wiley & Sons
external identifiers
  • wos:000279801000004
  • scopus:77953888794
ISSN
1099-1573
DOI
10.1002/ptr.3026
language
English
LU publication?
yes
id
6cfaadf5-5c51-446f-8aa0-b61c76bf7524 (old id 1656959)
date added to LUP
2010-08-25 11:27:00
date last changed
2018-05-29 11:30:10
@article{6cfaadf5-5c51-446f-8aa0-b61c76bf7524,
  abstract     = {Osteoporosis is a widespread problem afflicting millions of people. Drynol Cibotinis is a newly developed proprietary botanical combination of eight botanicals including Angelica sinensis, Glycine max, Wild yam, Ligustrum lucidum, Astragalus membranaceus, Cuscuta chinensis, Psoraleae corylifoliae, and Drynaria fortune. Each of the botanicals has been used in traditional Chinese medicine to treat osteoporosis. The effect of Drynol Cibotinis, with the specific combination of these anti-osteoporosis botanicals for promoting bone growth, was examined in this study. The effects of Drynol Cibotin on cell growth, apoptosis, cell spreading, calcium uptake and production of bone matrix proteins Collagen I and Laminin B2 on human osteoblast cells were assessed by BrdU incorporation, TUNEL assay, cell staining, intracellular Ca2+ measurement and Western blot analysis. The results showed that Drynol Cibotin significantly increased cell proliferation and inhibited apoptosis in osteoblasts (P &lt; 0.01). In addition, Drynol Cibotin was found to promote cell spreading and greatly increase calcium uptake both instantaneously and in the long term (P &lt; 0.01). Furthermore, Drynol Cibotin significantly increased production of two key extracellular matrix proteins in bone cells: Collagen I and Laminin B2. These results indicate that Drynol Cibotin alone or in combination with amino acids and vitamins may have prophylactic potentials in osteoporosis. Copyright (C) 2009 John Wiley &amp; Sons, Ltd.},
  author       = {Wegiel, Barbara and Persson, Jenny L},
  issn         = {1099-1573},
  keyword      = {Drynol Cibotin,collagen,calcium,bone,osteoporosis},
  language     = {eng},
  pages        = {139--147},
  publisher    = {John Wiley & Sons},
  series       = {Phytotherapy Research},
  title        = {Effect of a Novel Botanical Agent Drynol Cibotin on Human Osteoblast Cells and Implications for Osteoporosis: Promotion of Cell Growth, Calcium Uptake and Collagen Production},
  url          = {http://dx.doi.org/10.1002/ptr.3026},
  volume       = {24},
  year         = {2010},
}