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Lipopolysaccharide-induced down-regulation of uteroglobin in the human nose.

Fransson, Mattias LU ; Adner, Mikael LU ; Uddman, Rolf LU and Cardell, Lars-Olaf LU (2007) In Acta Oto-Laryngologica 127(3). p.285-291
Abstract
Conclusion. Lipopolysaccharide (LPS) challenge of the human nose has the capacity to reduce the amount of natural anti-inflammatory proteins, such as uteroglobin. Objectives. Nasal challenge with LPS, an activator of innate immunity, has been shown to increase the amount of pro-inflammatory mediators in nasal lavage fluid. Uteroglobin is a newly described anti-inflammatory mediator that is secreted in the nose. This study examined the effect of nasal LPS application on the level of uteroglobin in nasal lavage fluid as well as on the expression of uteroglobin in nasal mucosa. Materials and methods. Thirty-eight volunteers were challenged nasally with either 50 mu g LPS or vehicle; 6 h later, nasal lavage fluid was collected and a nasal... (More)
Conclusion. Lipopolysaccharide (LPS) challenge of the human nose has the capacity to reduce the amount of natural anti-inflammatory proteins, such as uteroglobin. Objectives. Nasal challenge with LPS, an activator of innate immunity, has been shown to increase the amount of pro-inflammatory mediators in nasal lavage fluid. Uteroglobin is a newly described anti-inflammatory mediator that is secreted in the nose. This study examined the effect of nasal LPS application on the level of uteroglobin in nasal lavage fluid as well as on the expression of uteroglobin in nasal mucosa. Materials and methods. Thirty-eight volunteers were challenged nasally with either 50 mu g LPS or vehicle; 6 h later, nasal lavage fluid was collected and a nasal biopsy was obtained. Levels of uteroglobin, albumin and the pro-inflammatory mediators interleukin (IL)-6 and IL-8 were analysed in the lavage fluids using enzyme-linked immunosorbent assays (ELISAs). Biopsies were used for either quantification of uteroglobin mRNA by real-time PCR or for localization of the corresponding protein with immunohistochemistry. Results. The uteroglobin level decreased in nasal lavage fluid following LPS challenge, whereas the levels of IL-6 and albumin increased. Uteroglobin was mainly seen in the respiratory epithelium and its mRNA expression decreased as a consequence of the LPS challenge. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lavage, nasal, mediator, innate immunity, Clara cell protein 16, endotoxin, mucosa
in
Acta Oto-Laryngologica
volume
127
issue
3
pages
285 - 291
publisher
Taylor & Francis
external identifiers
  • wos:000244695400010
  • scopus:33847190247
ISSN
1651-2251
DOI
10.1080/00016480600801340
language
English
LU publication?
yes
id
c93b4739-6a3a-45a5-b960-bd6034385b57 (old id 166490)
date added to LUP
2007-07-19 11:21:58
date last changed
2017-10-01 04:50:05
@article{c93b4739-6a3a-45a5-b960-bd6034385b57,
  abstract     = {Conclusion. Lipopolysaccharide (LPS) challenge of the human nose has the capacity to reduce the amount of natural anti-inflammatory proteins, such as uteroglobin. Objectives. Nasal challenge with LPS, an activator of innate immunity, has been shown to increase the amount of pro-inflammatory mediators in nasal lavage fluid. Uteroglobin is a newly described anti-inflammatory mediator that is secreted in the nose. This study examined the effect of nasal LPS application on the level of uteroglobin in nasal lavage fluid as well as on the expression of uteroglobin in nasal mucosa. Materials and methods. Thirty-eight volunteers were challenged nasally with either 50 mu g LPS or vehicle; 6 h later, nasal lavage fluid was collected and a nasal biopsy was obtained. Levels of uteroglobin, albumin and the pro-inflammatory mediators interleukin (IL)-6 and IL-8 were analysed in the lavage fluids using enzyme-linked immunosorbent assays (ELISAs). Biopsies were used for either quantification of uteroglobin mRNA by real-time PCR or for localization of the corresponding protein with immunohistochemistry. Results. The uteroglobin level decreased in nasal lavage fluid following LPS challenge, whereas the levels of IL-6 and albumin increased. Uteroglobin was mainly seen in the respiratory epithelium and its mRNA expression decreased as a consequence of the LPS challenge.},
  author       = {Fransson, Mattias and Adner, Mikael and Uddman, Rolf and Cardell, Lars-Olaf},
  issn         = {1651-2251},
  keyword      = {lavage,nasal,mediator,innate immunity,Clara cell protein 16,endotoxin,mucosa},
  language     = {eng},
  number       = {3},
  pages        = {285--291},
  publisher    = {Taylor & Francis},
  series       = {Acta Oto-Laryngologica},
  title        = {Lipopolysaccharide-induced down-regulation of uteroglobin in the human nose.},
  url          = {http://dx.doi.org/10.1080/00016480600801340},
  volume       = {127},
  year         = {2007},
}