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Nano-engineered living bacterial motors for active microfluidic mixing.

Al-Fandi, M; Jaradat, M A K; Fandi, K; Beech, Jason LU ; Tegenfeldt, Jonas LU and Yih, T C (2010) In IET Nanobiotechnology 4(3). p.61-71
Abstract
Active micromixers with rotating elements are attractive microfluidic actuators in many applications because of their mixing ability at a short distance. However, miniaturising the impeller design poses technical challenges including the fabrication and driving means. As a possible solution inspired by macro magnetic bar-stirrers, this study proposes the use of tethered, rotating bacteria as mixing elements. A tethered cell is a genetically engineered, harmless Escherichia coli (E. coli) attached to a surface by a single, shortened flagellum. The tethered flagellum acts as a pivot around which the entire cell body smoothly rotates. Videomicroscopy, image analysis and computational fluid dynamics (CFD) are utilised to demonstrate a... (More)
Active micromixers with rotating elements are attractive microfluidic actuators in many applications because of their mixing ability at a short distance. However, miniaturising the impeller design poses technical challenges including the fabrication and driving means. As a possible solution inspired by macro magnetic bar-stirrers, this study proposes the use of tethered, rotating bacteria as mixing elements. A tethered cell is a genetically engineered, harmless Escherichia coli (E. coli) attached to a surface by a single, shortened flagellum. The tethered flagellum acts as a pivot around which the entire cell body smoothly rotates. Videomicroscopy, image analysis and computational fluid dynamics (CFD) are utilised to demonstrate a proof-of-concept for the micro mixing process. Flow visualisation experiments show that a approximately 3 [micro sign]m long tethered E. coli rotating at approximately 240 rpm can circulate a 1 [micro sign]m polystyrene bead in the adjacent area at an average speed of nearly 4 [micro sign]m/s. The Peclet (Pe(b)) number for the stirred bead is evaluated to approximately 4. CFD simulations show that the rotary motion of a tethered E. coli rotating at 240 rpm can generate fluid velocities, up to 37 [micro sign]m/s bordering the cell envelop. Based on these simulations, the Strouhal number (St) is calculated to about 2. This hybrid bio-inorganic micromxer could be used as a local, disposable mixer. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
IET Nanobiotechnology
volume
4
issue
3
pages
61 - 71
publisher
Institution of Engineering and Technology
external identifiers
  • wos:000281000600002
  • pmid:20726672
  • scopus:77956054713
ISSN
1751-875X
DOI
10.1049/iet-nbt.2010.0003
language
English
LU publication?
yes
id
f576480b-9eab-4498-9a2b-baab4f58fec4 (old id 1665160)
date added to LUP
2010-09-08 15:46:32
date last changed
2018-05-29 10:24:40
@article{f576480b-9eab-4498-9a2b-baab4f58fec4,
  abstract     = {Active micromixers with rotating elements are attractive microfluidic actuators in many applications because of their mixing ability at a short distance. However, miniaturising the impeller design poses technical challenges including the fabrication and driving means. As a possible solution inspired by macro magnetic bar-stirrers, this study proposes the use of tethered, rotating bacteria as mixing elements. A tethered cell is a genetically engineered, harmless Escherichia coli (E. coli) attached to a surface by a single, shortened flagellum. The tethered flagellum acts as a pivot around which the entire cell body smoothly rotates. Videomicroscopy, image analysis and computational fluid dynamics (CFD) are utilised to demonstrate a proof-of-concept for the micro mixing process. Flow visualisation experiments show that a approximately 3 [micro sign]m long tethered E. coli rotating at approximately 240 rpm can circulate a 1 [micro sign]m polystyrene bead in the adjacent area at an average speed of nearly 4 [micro sign]m/s. The Peclet (Pe(b)) number for the stirred bead is evaluated to approximately 4. CFD simulations show that the rotary motion of a tethered E. coli rotating at 240 rpm can generate fluid velocities, up to 37 [micro sign]m/s bordering the cell envelop. Based on these simulations, the Strouhal number (St) is calculated to about 2. This hybrid bio-inorganic micromxer could be used as a local, disposable mixer.},
  author       = {Al-Fandi, M and Jaradat, M A K and Fandi, K and Beech, Jason and Tegenfeldt, Jonas and Yih, T C},
  issn         = {1751-875X},
  language     = {eng},
  number       = {3},
  pages        = {61--71},
  publisher    = {Institution of Engineering and Technology},
  series       = {IET Nanobiotechnology},
  title        = {Nano-engineered living bacterial motors for active microfluidic mixing.},
  url          = {http://dx.doi.org/10.1049/iet-nbt.2010.0003},
  volume       = {4},
  year         = {2010},
}