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Potential effects of PKC or protease inhibitors on acute pancreatitis-induced tissue injury in rats.

Shi, Changbin LU ; Zhao, Xia LU ; Wang, Xiangdong; Zhao, Liming and Andersson, Roland LU (2007) In Vascular Pharmacology 46(6). p.406-411
Abstract
Background: Acute pancreatitis (AP) is still one of the severe diseases, that cause the development of multiple organ dysfunction with a high mortality. Effective therapies for AP are still limited, mainly due to unclear mechanisms by which A-P initiates both pancreatic and extrapancreatic organ injury. Methods: Protease inhibitors (aprotinin, pefabloc, trypsin inhibitor) and PKC inhibitors (polymyxin B, staurosporine) were administrated 30 min before 'induction of AP in rats. To investigate the pancreatic, systemic and lung inflammatory response and injury, plasma IL-6 and IL-10, pancreatic and pulmonary myeloperoxidase (NIPO) levels, pancreatic protease activity and phospholipase A(2) (PLA(2)) activity in ascites were measured 3 and 6 h... (More)
Background: Acute pancreatitis (AP) is still one of the severe diseases, that cause the development of multiple organ dysfunction with a high mortality. Effective therapies for AP are still limited, mainly due to unclear mechanisms by which A-P initiates both pancreatic and extrapancreatic organ injury. Methods: Protease inhibitors (aprotinin, pefabloc, trypsin inhibitor) and PKC inhibitors (polymyxin B, staurosporine) were administrated 30 min before 'induction of AP in rats. To investigate the pancreatic, systemic and lung inflammatory response and injury, plasma IL-6 and IL-10, pancreatic and pulmonary myeloperoxidase (NIPO) levels, pancreatic protease activity and phospholipase A(2) (PLA(2)) activity in ascites were measured 3 and 6 h after AP induction. Results: Pretreatment with protease inhibitors significantly prevented from AP-increased plasma levels of IL-10, pancreatic and pulmonary levels of MTO, pancreatic protease activity and the catalytic activity of PLA(2) in ascites. PKC inhibitors significantly reduced pancreatic and pulmonary levels of MTO and pancreatic protease activity. Conclusion: Inhibition of proteases in AP may be helpful in ameliorating the inflammatory reaction in both pancreatic and extrapancreatic tissues, where neutrophil involvement may be regulated by PKC and proteases. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acute pancreatitis, protease, protein kinase C, inhibitors
in
Vascular Pharmacology
volume
46
issue
6
pages
406 - 411
publisher
Elsevier
external identifiers
  • wos:000246333600003
  • scopus:34047266356
ISSN
1537-1891
DOI
10.1016/j.vph.2007.01.009
language
English
LU publication?
yes
id
8a365323-5c28-457f-8e6e-3095e83f3f5d (old id 166705)
date added to LUP
2007-07-24 09:54:09
date last changed
2017-01-01 04:27:59
@article{8a365323-5c28-457f-8e6e-3095e83f3f5d,
  abstract     = {Background: Acute pancreatitis (AP) is still one of the severe diseases, that cause the development of multiple organ dysfunction with a high mortality. Effective therapies for AP are still limited, mainly due to unclear mechanisms by which A-P initiates both pancreatic and extrapancreatic organ injury. Methods: Protease inhibitors (aprotinin, pefabloc, trypsin inhibitor) and PKC inhibitors (polymyxin B, staurosporine) were administrated 30 min before 'induction of AP in rats. To investigate the pancreatic, systemic and lung inflammatory response and injury, plasma IL-6 and IL-10, pancreatic and pulmonary myeloperoxidase (NIPO) levels, pancreatic protease activity and phospholipase A(2) (PLA(2)) activity in ascites were measured 3 and 6 h after AP induction. Results: Pretreatment with protease inhibitors significantly prevented from AP-increased plasma levels of IL-10, pancreatic and pulmonary levels of MTO, pancreatic protease activity and the catalytic activity of PLA(2) in ascites. PKC inhibitors significantly reduced pancreatic and pulmonary levels of MTO and pancreatic protease activity. Conclusion: Inhibition of proteases in AP may be helpful in ameliorating the inflammatory reaction in both pancreatic and extrapancreatic tissues, where neutrophil involvement may be regulated by PKC and proteases.},
  author       = {Shi, Changbin and Zhao, Xia and Wang, Xiangdong and Zhao, Liming and Andersson, Roland},
  issn         = {1537-1891},
  keyword      = {acute pancreatitis,protease,protein kinase C,inhibitors},
  language     = {eng},
  number       = {6},
  pages        = {406--411},
  publisher    = {Elsevier},
  series       = {Vascular Pharmacology},
  title        = {Potential effects of PKC or protease inhibitors on acute pancreatitis-induced tissue injury in rats.},
  url          = {http://dx.doi.org/10.1016/j.vph.2007.01.009},
  volume       = {46},
  year         = {2007},
}