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Expression of Toll-like receptor 9 in nose, peripheral blood and bone marrow during symptomatic allergic rhinitis.

Fransson, Mattias LU ; Benson, Mikael; Erjefält, Jonas LU ; Jansson, Lennart; Uddman, Rolf LU ; Björnsson, Sven LU ; Cardell, Lars-Olaf LU and Adner, Mikael LU (2007) In Respiratory Research 8(17). p.17-17
Abstract
BACKGROUND: Allergic rhinitis is an inflammatory disease of the upper airway mucosa that also affects leukocytes in bone marrow and peripheral blood. Toll-like receptor 9 (TLR9) is a receptor for unmethylated CpG dinucleotides found in bacterial and viral DNA. The present study was designed to examine the expression of TLR9 in the nasal mucosa and in leukocytes derived from different cellular compartments during symptomatic allergic rhinitis. METHODS: The study was based on 32 patients with seasonal allergic rhinitis and 18 healthy subjects, serving as controls. Nasal biopsies were obtained before and after allergen challenge. Bone marrow, peripheral blood and nasal lavage fluid were sampled outside and during pollen season. The expression... (More)
BACKGROUND: Allergic rhinitis is an inflammatory disease of the upper airway mucosa that also affects leukocytes in bone marrow and peripheral blood. Toll-like receptor 9 (TLR9) is a receptor for unmethylated CpG dinucleotides found in bacterial and viral DNA. The present study was designed to examine the expression of TLR9 in the nasal mucosa and in leukocytes derived from different cellular compartments during symptomatic allergic rhinitis. METHODS: The study was based on 32 patients with seasonal allergic rhinitis and 18 healthy subjects, serving as controls. Nasal biopsies were obtained before and after allergen challenge. Bone marrow, peripheral blood and nasal lavage fluid were sampled outside and during pollen season. The expression of TLR9 in tissues and cells was analyzed using immunohistochemistry and flow cytometry, respectively. RESULTS: TLR9 was found in several cell types in the nasal mucosa and in different leukocyte subpopulations derived from bone marrow, peripheral blood and nasal lavage fluid. The leukocyte expression was generally higher in bone marrow than in peripheral blood, and not affected by symptomatic allergic rhinitis. CONCLUSION: The widespread expression of TLR9 in the nasal mucosa along with its rich representation in leukocytes in different compartments, demonstrate the possibility for cells involved in allergic airway inflammation to directly interact with bacterial and viral DNA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Middle Aged, Male, Leukocytes: metabolism, Humans, Female, Bone Marrow: metabolism, Biological Markers: metabolism, Nasal Mucosa: metabolism, Rhinitis, Adult, Allergic, Adolescent, Seasonal: metabolism, Toll-Like Receptor 9: metabolism
in
Respiratory Research
volume
8
issue
17
pages
17 - 17
publisher
BioMed Central
external identifiers
  • wos:000244744600002
  • scopus:42449119776
ISSN
1465-9921
DOI
10.1186/1465-9921-8-17
language
English
LU publication?
yes
id
1d0f5b4a-0c43-4371-88ed-84b145a18b71 (old id 166959)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17328813&dopt=Abstract
date added to LUP
2007-07-12 11:10:45
date last changed
2017-03-26 03:36:43
@article{1d0f5b4a-0c43-4371-88ed-84b145a18b71,
  abstract     = {BACKGROUND: Allergic rhinitis is an inflammatory disease of the upper airway mucosa that also affects leukocytes in bone marrow and peripheral blood. Toll-like receptor 9 (TLR9) is a receptor for unmethylated CpG dinucleotides found in bacterial and viral DNA. The present study was designed to examine the expression of TLR9 in the nasal mucosa and in leukocytes derived from different cellular compartments during symptomatic allergic rhinitis. METHODS: The study was based on 32 patients with seasonal allergic rhinitis and 18 healthy subjects, serving as controls. Nasal biopsies were obtained before and after allergen challenge. Bone marrow, peripheral blood and nasal lavage fluid were sampled outside and during pollen season. The expression of TLR9 in tissues and cells was analyzed using immunohistochemistry and flow cytometry, respectively. RESULTS: TLR9 was found in several cell types in the nasal mucosa and in different leukocyte subpopulations derived from bone marrow, peripheral blood and nasal lavage fluid. The leukocyte expression was generally higher in bone marrow than in peripheral blood, and not affected by symptomatic allergic rhinitis. CONCLUSION: The widespread expression of TLR9 in the nasal mucosa along with its rich representation in leukocytes in different compartments, demonstrate the possibility for cells involved in allergic airway inflammation to directly interact with bacterial and viral DNA.},
  author       = {Fransson, Mattias and Benson, Mikael and Erjefält, Jonas and Jansson, Lennart and Uddman, Rolf and Björnsson, Sven and Cardell, Lars-Olaf and Adner, Mikael},
  issn         = {1465-9921},
  keyword      = {Middle Aged,Male,Leukocytes: metabolism,Humans,Female,Bone Marrow: metabolism,Biological Markers: metabolism,Nasal Mucosa: metabolism,Rhinitis,Adult,Allergic,Adolescent,Seasonal: metabolism,Toll-Like Receptor 9: metabolism},
  language     = {eng},
  number       = {17},
  pages        = {17--17},
  publisher    = {BioMed Central},
  series       = {Respiratory Research},
  title        = {Expression of Toll-like receptor 9 in nose, peripheral blood and bone marrow during symptomatic allergic rhinitis.},
  url          = {http://dx.doi.org/10.1186/1465-9921-8-17},
  volume       = {8},
  year         = {2007},
}