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Identification of prostate specific antigen (PSA) isoforms in complex biological samples utilizing complementary platforms

Végvári, Ákos LU ; Rezeli, Melinda LU ; Welinder, Charlotte LU ; Malm, Johan LU ; Lilja, Hans LU ; Marko-Varga, György LU and Laurell, Thomas LU (2010) In Journal of Proteomics2008-01-01+01:00 73(6). p.1137-1147
Abstract
Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.

Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for... (More)
Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.

Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for this multiple step analytical approach to identify multiple PSA variants from complex biological samples that revealed distinct molecular characteristics. In addition, sequence-annotated protein bands in SDS–PAGE gels were compared to those detected by Western blots, and by monitoring the enzymatic activity in zymogram gels, using gelatin as a substrate. The high accuracy annotations were obtained by fast turnaround MALDI-Orbitrap analysis from excised and digested gel bands. Multiple PSA forms were identified utilizing a combination of MASCOT and SEQUEST search engines. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Seminal plasma, Mass spectrometry, Prostate-specific antigen isoforms, MALDI LTQ Orbitrap XL
in
Journal of Proteomics2008-01-01+01:00
volume
73
issue
6
pages
1137 - 1147
publisher
Elsevier
external identifiers
  • wos:000277763800011
  • scopus:77950518665
ISSN
1874-3919
DOI
10.1016/j.jprot.2010.01.008
language
English
LU publication?
yes
id
0a442c76-4d8b-46cc-8c3f-0dddac561f7a (old id 1670529)
date added to LUP
2010-09-14 14:29:44
date last changed
2018-05-29 11:33:12
@article{0a442c76-4d8b-46cc-8c3f-0dddac561f7a,
  abstract     = {Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.<br/><br>
Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for this multiple step analytical approach to identify multiple PSA variants from complex biological samples that revealed distinct molecular characteristics. In addition, sequence-annotated protein bands in SDS–PAGE gels were compared to those detected by Western blots, and by monitoring the enzymatic activity in zymogram gels, using gelatin as a substrate. The high accuracy annotations were obtained by fast turnaround MALDI-Orbitrap analysis from excised and digested gel bands. Multiple PSA forms were identified utilizing a combination of MASCOT and SEQUEST search engines.},
  author       = {Végvári, Ákos and Rezeli, Melinda and Welinder, Charlotte and Malm, Johan and Lilja, Hans and Marko-Varga, György and Laurell, Thomas},
  issn         = {1874-3919},
  keyword      = {Seminal plasma,Mass spectrometry,Prostate-specific antigen isoforms,MALDI LTQ Orbitrap XL},
  language     = {eng},
  number       = {6},
  pages        = {1137--1147},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics2008-01-01+01:00},
  title        = {Identification of prostate specific antigen (PSA) isoforms in complex biological samples utilizing complementary platforms},
  url          = {http://dx.doi.org/10.1016/j.jprot.2010.01.008},
  volume       = {73},
  year         = {2010},
}