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Impaired opsonization with complement and phagocytosis of Streptococcus pyogenes in sera from subjects with inherited C2 deficiency

Yuste, Jose; Sen, Ashwin; Truedsson, Lennart LU ; Jönsson, Göran LU ; Hyams, Catherine; Cohen, Jonathan M.; Camberlein, Emilie; Sriskandan, Shiranee and Brown, Jeremy S. (2010) In Microbes and Infection 12(8-9). p.626-634
Abstract
Although subjects with inherited defects of the classical complement pathway component C2 are at increased risk of infection, there are few experimental data available on which bacterial pathogens they might be susceptible to. In order to investigate whether patients with inherited C2 deficiency may have increased susceptibility to Streptococcus pyogenes infection we have analysed opsonization with C3b/iC3b and phagocytosis of three different strains of S. pyogenes in serum from 8 C2(-/-) subjects using flow cytometry assays. Sera from patients with C2 deficiency had a markedly reduced ability to opsonise S. pyogenes with C3b/iC3b. In addition, phagocytosis of all three S. pyogenes strains was impaired in sera from C2(-/-) subjects. Both... (More)
Although subjects with inherited defects of the classical complement pathway component C2 are at increased risk of infection, there are few experimental data available on which bacterial pathogens they might be susceptible to. In order to investigate whether patients with inherited C2 deficiency may have increased susceptibility to Streptococcus pyogenes infection we have analysed opsonization with C3b/iC3b and phagocytosis of three different strains of S. pyogenes in serum from 8 C2(-/-) subjects using flow cytometry assays. Sera from patients with C2 deficiency had a markedly reduced ability to opsonise S. pyogenes with C3b/iC3b. In addition, phagocytosis of all three S. pyogenes strains was impaired in sera from C2(-/-) subjects. Both the reduced opsonisation with C3b/iC3b and phagocytosis in C2(-/-) sera were markedly improved by addition of exogenous C2 protein. Neutrophil dependent killing was also reduced, confirming the functional importance of C2 deficiency for immunity to S. pyogenes. Impaired opsonisation with C3b/iC3b and phagocytosis was not related to reduced recognition of the bacteria by antibody. These data suggest that patients with C2 deficiency are at increased risk of S. pyogenes infections. (c) 2010 Elsevier Masson SAS. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phagocytosis, Complement, C2 deficiency, Streptococcus pyogenes
in
Microbes and Infection
volume
12
issue
8-9
pages
626 - 634
publisher
Elsevier
external identifiers
  • wos:000280780100005
  • scopus:77954955902
ISSN
1769-714X
DOI
10.1016/j.micinf.2010.04.004
language
English
LU publication?
yes
id
f77df645-3083-4dc2-aca6-1e26331010eb (old id 1675362)
date added to LUP
2010-09-22 12:45:25
date last changed
2018-05-29 10:39:40
@article{f77df645-3083-4dc2-aca6-1e26331010eb,
  abstract     = {Although subjects with inherited defects of the classical complement pathway component C2 are at increased risk of infection, there are few experimental data available on which bacterial pathogens they might be susceptible to. In order to investigate whether patients with inherited C2 deficiency may have increased susceptibility to Streptococcus pyogenes infection we have analysed opsonization with C3b/iC3b and phagocytosis of three different strains of S. pyogenes in serum from 8 C2(-/-) subjects using flow cytometry assays. Sera from patients with C2 deficiency had a markedly reduced ability to opsonise S. pyogenes with C3b/iC3b. In addition, phagocytosis of all three S. pyogenes strains was impaired in sera from C2(-/-) subjects. Both the reduced opsonisation with C3b/iC3b and phagocytosis in C2(-/-) sera were markedly improved by addition of exogenous C2 protein. Neutrophil dependent killing was also reduced, confirming the functional importance of C2 deficiency for immunity to S. pyogenes. Impaired opsonisation with C3b/iC3b and phagocytosis was not related to reduced recognition of the bacteria by antibody. These data suggest that patients with C2 deficiency are at increased risk of S. pyogenes infections. (c) 2010 Elsevier Masson SAS. All rights reserved.},
  author       = {Yuste, Jose and Sen, Ashwin and Truedsson, Lennart and Jönsson, Göran and Hyams, Catherine and Cohen, Jonathan M. and Camberlein, Emilie and Sriskandan, Shiranee and Brown, Jeremy S.},
  issn         = {1769-714X},
  keyword      = {Phagocytosis,Complement,C2 deficiency,Streptococcus pyogenes},
  language     = {eng},
  number       = {8-9},
  pages        = {626--634},
  publisher    = {Elsevier},
  series       = {Microbes and Infection},
  title        = {Impaired opsonization with complement and phagocytosis of Streptococcus pyogenes in sera from subjects with inherited C2 deficiency},
  url          = {http://dx.doi.org/10.1016/j.micinf.2010.04.004},
  volume       = {12},
  year         = {2010},
}