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Interactions between DNA and Nonionic Ethylene Oxide Surfactants are Predominantly Repulsive

Machado, Alexandra LU ; Lundberg, Dan LU ; Ribeiro, Antonio J.; Veiga, Francisco J.; Miguel, Maria G.; Lindman, Björn LU and Olsson, Ulf LU (2010) In Langmuir 26(16). p.13102-13109
Abstract
In the present work, the interactions between double-stranded (ds) or single-stranded (ss) DNA and nonionic ethylene oxide (EO) surfactants, with special attention to the possible contributions from hydrophobic interactions, have been investigated using a multitechnique approach. It was found that the presence of ss as well as dsDNA induces a slight decrease of the cloud point of pentaethylene glycol monododecyl ether (C12E5). Assessment of the partitioning of DNA between the surfactant-rich and surfactant-poor phases formed above the cloud point showed that the polymer was preferably located in the surfactant-poor phase. Surface tcnsiometry experiments revealed that neither of the DNA forms induced surfactant micellization. Finally, it... (More)
In the present work, the interactions between double-stranded (ds) or single-stranded (ss) DNA and nonionic ethylene oxide (EO) surfactants, with special attention to the possible contributions from hydrophobic interactions, have been investigated using a multitechnique approach. It was found that the presence of ss as well as dsDNA induces a slight decrease of the cloud point of pentaethylene glycol monododecyl ether (C12E5). Assessment of the partitioning of DNA between the surfactant-rich and surfactant-poor phases formed above the cloud point showed that the polymer was preferably located in the surfactant-poor phase. Surface tcnsiometry experiments revealed that neither of the DNA forms induced surfactant micellization. Finally, it was shown by DNA melting measurements that another EO surfactant (C12E8) did not affect the relative stabilities of ss and dsDNA. To summarize, all experiments suggest that the net interaction between DNA and nonionic surfactants of the EO type is weakly repulsive, which can be attributed mainly to steric effects. In general, the results were practically identical for the ds and ss forms of DNA, except those from the cloud point experiments, where the decrease of the cloud point was less pronounced with ssDNA. This finding indicates the presence of an attractive component in the interaction, which can reasonably be ascribed to hydrophobic effects. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
26
issue
16
pages
13102 - 13109
publisher
The American Chemical Society
external identifiers
  • wos:000280667900016
  • scopus:77955527632
ISSN
0743-7463
DOI
10.1021/la101403j
language
English
LU publication?
yes
id
8fba138d-e971-4044-a0dc-d0a0be10714c (old id 1675377)
date added to LUP
2010-09-22 12:56:14
date last changed
2018-06-10 03:27:26
@article{8fba138d-e971-4044-a0dc-d0a0be10714c,
  abstract     = {In the present work, the interactions between double-stranded (ds) or single-stranded (ss) DNA and nonionic ethylene oxide (EO) surfactants, with special attention to the possible contributions from hydrophobic interactions, have been investigated using a multitechnique approach. It was found that the presence of ss as well as dsDNA induces a slight decrease of the cloud point of pentaethylene glycol monododecyl ether (C12E5). Assessment of the partitioning of DNA between the surfactant-rich and surfactant-poor phases formed above the cloud point showed that the polymer was preferably located in the surfactant-poor phase. Surface tcnsiometry experiments revealed that neither of the DNA forms induced surfactant micellization. Finally, it was shown by DNA melting measurements that another EO surfactant (C12E8) did not affect the relative stabilities of ss and dsDNA. To summarize, all experiments suggest that the net interaction between DNA and nonionic surfactants of the EO type is weakly repulsive, which can be attributed mainly to steric effects. In general, the results were practically identical for the ds and ss forms of DNA, except those from the cloud point experiments, where the decrease of the cloud point was less pronounced with ssDNA. This finding indicates the presence of an attractive component in the interaction, which can reasonably be ascribed to hydrophobic effects.},
  author       = {Machado, Alexandra and Lundberg, Dan and Ribeiro, Antonio J. and Veiga, Francisco J. and Miguel, Maria G. and Lindman, Björn and Olsson, Ulf},
  issn         = {0743-7463},
  language     = {eng},
  number       = {16},
  pages        = {13102--13109},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Interactions between DNA and Nonionic Ethylene Oxide Surfactants are Predominantly Repulsive},
  url          = {http://dx.doi.org/10.1021/la101403j},
  volume       = {26},
  year         = {2010},
}