Proteomic analysis of striatal proteins in the rat model of l-DOPA-induced dyskinesia.
Valastro, Barbara LU ; Dekundy, Andrzej ; Krogh, Morten LU ; Lundblad, Martin LU ; James, Peter LU
; Danysz, Wojciech
; Quack, Guenter
and Cenci Nilsson, Angela
LU
(2007)
In Journal of Neurochemistry
102(4).
p.1395-1409
- Abstract
- L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for... (More)
- L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for statistical difference, 67 spots were significantly changed in abundance and identified using matrix-assisted laser desorption/ionization time-of-flight MS, atmospheric pressure matrix-assisted laser desorption/ionization and HPLC coupled tandem MS (LC/MS/ MS). Out of these 67 proteins, LID significantly changed the expression level of five proteins: alpha beta-crystalin, gamma-enolase, guanicloacetate methyltransferase, vinculin, and proteasome alpha-2 subunit. Complementary techniques such as western immunoblotting and immunohistochernistry were performed to investigate the validity of the data obtained using the proteomic approach. In conclusion, this study provides new insights into the protein changes occurring in LID. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/168014
- author
- Valastro, Barbara
LU
; Dekundy, Andrzej
; Krogh, Morten
LU
; Lundblad, Martin
LU
; James, Peter
LU
; Danysz, Wojciech
; Quack, Guenter
and Cenci Nilsson, Angela
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- spectrometry, two-dimensional difference gel electrophoresis, Parkinson's disease, mass, levodopa, dyskinesia, bromocriptine
- in
- Journal of Neurochemistry
- volume
- 102
- issue
- 4
- pages
- 1395 - 1409
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000248884100035
- scopus:34547467205
- pmid:17532790
- ISSN
- 1471-4159
- DOI
- 10.1111/j.1471-4159.2007.04655.x
- language
- English
- LU publication?
- yes
- id
- 11bf10ca-7790-4c42-a031-417ad801f36a (old id 168014)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17532790&dopt=Abstract
- date added to LUP
- 2016-04-01 16:33:19
- date last changed
- 2024-01-11 10:11:03
@article{11bf10ca-7790-4c42-a031-417ad801f36a,
abstract = {{L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for statistical difference, 67 spots were significantly changed in abundance and identified using matrix-assisted laser desorption/ionization time-of-flight MS, atmospheric pressure matrix-assisted laser desorption/ionization and HPLC coupled tandem MS (LC/MS/ MS). Out of these 67 proteins, LID significantly changed the expression level of five proteins: alpha beta-crystalin, gamma-enolase, guanicloacetate methyltransferase, vinculin, and proteasome alpha-2 subunit. Complementary techniques such as western immunoblotting and immunohistochernistry were performed to investigate the validity of the data obtained using the proteomic approach. In conclusion, this study provides new insights into the protein changes occurring in LID.}},
author = {{Valastro, Barbara and Dekundy, Andrzej and Krogh, Morten and Lundblad, Martin and James, Peter and Danysz, Wojciech and Quack, Guenter and Cenci Nilsson, Angela}},
issn = {{1471-4159}},
keywords = {{spectrometry; two-dimensional difference gel electrophoresis; Parkinson's disease; mass; levodopa; dyskinesia; bromocriptine}},
language = {{eng}},
number = {{4}},
pages = {{1395--1409}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Neurochemistry}},
title = {{Proteomic analysis of striatal proteins in the rat model of l-DOPA-induced dyskinesia.}},
url = {{http://dx.doi.org/10.1111/j.1471-4159.2007.04655.x}},
doi = {{10.1111/j.1471-4159.2007.04655.x}},
volume = {{102}},
year = {{2007}},
}