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Proteomic analysis of striatal proteins in the rat model of l-DOPA-induced dyskinesia.

Valastro, Barbara LU ; Dekundy, Andrzej; Krogh, Morten LU ; Lundblad, Martin LU ; James, Peter LU ; Danysz, Wojciech; Quack, Guenter and Cenci Nilsson, Angela LU (2007) In Journal of Neurochemistry 102(4). p.1395-1409
Abstract
L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for... (More)
L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for statistical difference, 67 spots were significantly changed in abundance and identified using matrix-assisted laser desorption/ionization time-of-flight MS, atmospheric pressure matrix-assisted laser desorption/ionization and HPLC coupled tandem MS (LC/MS/ MS). Out of these 67 proteins, LID significantly changed the expression level of five proteins: alpha beta-crystalin, gamma-enolase, guanicloacetate methyltransferase, vinculin, and proteasome alpha-2 subunit. Complementary techniques such as western immunoblotting and immunohistochernistry were performed to investigate the validity of the data obtained using the proteomic approach. In conclusion, this study provides new insights into the protein changes occurring in LID. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
spectrometry, two-dimensional difference gel electrophoresis, Parkinson's disease, mass, levodopa, dyskinesia, bromocriptine
in
Journal of Neurochemistry
volume
102
issue
4
pages
1395 - 1409
publisher
Wiley-Blackwell
external identifiers
  • wos:000248884100035
  • scopus:34547467205
ISSN
1471-4159
DOI
10.1111/j.1471-4159.2007.04655.x
language
English
LU publication?
yes
id
11bf10ca-7790-4c42-a031-417ad801f36a (old id 168014)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17532790&dopt=Abstract
date added to LUP
2007-07-20 08:16:09
date last changed
2017-07-30 04:38:27
@article{11bf10ca-7790-4c42-a031-417ad801f36a,
  abstract     = {L-DOPA-induced dyskinesia (LID) is among the motor complications that arise in Parkinson's disease (PD) patients after a prolonged treatment with L-DOPA. To this day, transcriptome analysis has been performed in a rat model of LID [Neurobiol. Dis., 17 (2004), 219] but information regarding the proteome is still lacking. In the present study, we investigated the changes occurring at the protein level in striatal samples obtained from the unilaterally 6-hydroxydopamine-lesion rat model of PD treated with saline, L-DOPA or bromocriptine using two-dimensional difference gel electrophoresis and mass spectrometry (MS). Rats treated with L-DOPA were allocated to two groups based on the presence or absence of LID. Among the 2000 spots compared for statistical difference, 67 spots were significantly changed in abundance and identified using matrix-assisted laser desorption/ionization time-of-flight MS, atmospheric pressure matrix-assisted laser desorption/ionization and HPLC coupled tandem MS (LC/MS/ MS). Out of these 67 proteins, LID significantly changed the expression level of five proteins: alpha beta-crystalin, gamma-enolase, guanicloacetate methyltransferase, vinculin, and proteasome alpha-2 subunit. Complementary techniques such as western immunoblotting and immunohistochernistry were performed to investigate the validity of the data obtained using the proteomic approach. In conclusion, this study provides new insights into the protein changes occurring in LID.},
  author       = {Valastro, Barbara and Dekundy, Andrzej and Krogh, Morten and Lundblad, Martin and James, Peter and Danysz, Wojciech and Quack, Guenter and Cenci Nilsson, Angela},
  issn         = {1471-4159},
  keyword      = {spectrometry,two-dimensional difference gel electrophoresis,Parkinson's disease,mass,levodopa,dyskinesia,bromocriptine},
  language     = {eng},
  number       = {4},
  pages        = {1395--1409},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Proteomic analysis of striatal proteins in the rat model of l-DOPA-induced dyskinesia.},
  url          = {http://dx.doi.org/10.1111/j.1471-4159.2007.04655.x},
  volume       = {102},
  year         = {2007},
}