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Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.

Brune, Jan Claas LU ; Tormin, Ariane LU ; Johansson, Maria C LU ; Rissler, Pehr LU ; Brosjö, Otte; Löfvenberg, Richard; Vult von Steyern, Fredrik LU ; Mertens, Fredrik LU ; Rydholm, Anders LU and Scheding, Stefan LU (2011) In International Journal of Cancer 129. p.319-330
Abstract
Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5)... (More)
Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5) cells, n=8). OS-derived MSC (OS-MSC) showed normal MSC morphology and expressed the typical MSC surface marker profile (CD105/CD73/CD90/CD44/HLA-classI/CD166 positive, CD45/CD34/CD14/CD19/HLA-DR/CD31 negative). Furthermore, all OS-MSC samples could be differentiated into the osteogenic lineage, and all but one sample into adipocytes and chondrocytes. Genetic analysis of OS-MSC as well as OS-derived spheres showed no tumor-related chromosomal aberrations. OS-MSC expression of markers related to tumor-associated fibroblasts (fibroblast surface protein, alpha-smooth muscle actin, vimentin) was comparable to bone marrow MSC and OS-MSC growth was considerably affected by tyrosine kinase inhibitors. Taken together, our results demonstrate that normal, non-malignant mesenchymal stroma cells are isolated from OS when MSC culture techniques are applied. OS-MSC represent a major constituent of the tumor microenvironment, and they share many properties with bone marrow-derived MSC. © 2010 UICC. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mesenchymal stromal cells, osteosarcoma, osteogenic sarcoma, cancer associated fibroblasts, tumor stroma
in
International Journal of Cancer
volume
129
pages
319 - 330
publisher
John Wiley & Sons
external identifiers
  • wos:000291603900006
  • pmid:20878957
  • scopus:79961123373
ISSN
0020-7136
DOI
10.1002/ijc.25697
language
English
LU publication?
yes
id
e543d695-004d-4aa6-8edd-71c9479cbf1f (old id 1687657)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20878957?dopt=Abstract
date added to LUP
2010-10-07 15:33:42
date last changed
2017-08-13 03:23:29
@article{e543d695-004d-4aa6-8edd-71c9479cbf1f,
  abstract     = {Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5) cells, n=8). OS-derived MSC (OS-MSC) showed normal MSC morphology and expressed the typical MSC surface marker profile (CD105/CD73/CD90/CD44/HLA-classI/CD166 positive, CD45/CD34/CD14/CD19/HLA-DR/CD31 negative). Furthermore, all OS-MSC samples could be differentiated into the osteogenic lineage, and all but one sample into adipocytes and chondrocytes. Genetic analysis of OS-MSC as well as OS-derived spheres showed no tumor-related chromosomal aberrations. OS-MSC expression of markers related to tumor-associated fibroblasts (fibroblast surface protein, alpha-smooth muscle actin, vimentin) was comparable to bone marrow MSC and OS-MSC growth was considerably affected by tyrosine kinase inhibitors. Taken together, our results demonstrate that normal, non-malignant mesenchymal stroma cells are isolated from OS when MSC culture techniques are applied. OS-MSC represent a major constituent of the tumor microenvironment, and they share many properties with bone marrow-derived MSC. © 2010 UICC.},
  author       = {Brune, Jan Claas and Tormin, Ariane and Johansson, Maria C and Rissler, Pehr and Brosjö, Otte and Löfvenberg, Richard and Vult von Steyern, Fredrik and Mertens, Fredrik and Rydholm, Anders and Scheding, Stefan},
  issn         = {0020-7136},
  keyword      = {mesenchymal stromal cells,osteosarcoma,osteogenic sarcoma,cancer associated fibroblasts,tumor stroma},
  language     = {eng},
  pages        = {319--330},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.},
  url          = {http://dx.doi.org/10.1002/ijc.25697},
  volume       = {129},
  year         = {2011},
}