Isoforms diversity and transcriptional regulation of floral meristem identity and pathway integrators, driving floral transition in mango under subtropics
(2024) In Scientia Horticulturae 334.- Abstract
Floral initiation in mango is intricately regulated by a complex genetic network that integrates diverse environmental and endogenous cues. The flowering patterns in different varieties contingent upon their genetic network and sensitivity to the environmental signals, culminating in the phenomenon of "alternate bearing in several mango varieties. In this study, we conducted a comprehensive molecular dissection of the genetic regulators of flowering through comparative transcriptomics in mango varieties having contrast flowering pattern traits. A total of 45 and 54 million 150 base-paired reads were obtained and assembled in 58,094 and 50,945 transcripts for Amrapali and Chausa, respectively. Among them several candidates were... (More)
Floral initiation in mango is intricately regulated by a complex genetic network that integrates diverse environmental and endogenous cues. The flowering patterns in different varieties contingent upon their genetic network and sensitivity to the environmental signals, culminating in the phenomenon of "alternate bearing in several mango varieties. In this study, we conducted a comprehensive molecular dissection of the genetic regulators of flowering through comparative transcriptomics in mango varieties having contrast flowering pattern traits. A total of 45 and 54 million 150 base-paired reads were obtained and assembled in 58,094 and 50,945 transcripts for Amrapali and Chausa, respectively. Among them several candidates were identified as flowering regulators, operating at multilevel like floral meristem transition (SUPPRESSOR OF OVER-EXPRESSION OF CONSTANS, MiSOC1, and APETELA, MiAP1 and MiAP2), floral signal integrators (CONSTANS- LIKE, MiCOL; AGAMOUS LIKE MiAGL and SQUAMOSA PROMOTER BINDING PROTEIN LIKE, MiSPL), hormonal regulation (S-adenosyl methionine synthase, MiSAMS), cell division and cell cycle regulators (Cyclin dependant kinase, MiCDK5 and SIN-associated polypeptide, MiSAP18). Moreover, multiple isoforms of these flowering genes were also identified in these varieties. qRT-PCR analysis revealed differential transcriptional regulation of these multiple isoforms in floral and vegetative buds in the contrasting varieties. Furthermore, by investigating the gene expression of these multiple isoforms in various mango varieties with different flowering pattern traits, we established the role of these isoforms in mango flowering. Finally, gene expression findings supported by protein motif analysis and phylogenetic insights facilitated the development of a comprehensive flowering model that may applicable to subtropical ecology.
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- author
- Bajpai, Anju ; M․, Muthukumar ; Bajpai, Yashi ; Khan, Kasim LU ; Rajan, S. ; Singh, N. K. and Singh, V. K.
- organization
- publishing date
- 2024-08-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Floral induction, Floral meristem identity genes, Floral signal integrators, Isoforms diversity, Mango
- in
- Scientia Horticulturae
- volume
- 334
- article number
- 113301
- pages
- 15 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85193435689
- ISSN
- 0304-4238
- DOI
- 10.1016/j.scienta.2024.113301
- language
- English
- LU publication?
- yes
- id
- 16b8f88d-f690-4a70-aaf5-f36d1a3c0cf7
- date added to LUP
- 2024-05-31 10:15:51
- date last changed
- 2024-06-03 17:38:27
@article{16b8f88d-f690-4a70-aaf5-f36d1a3c0cf7,
abstract = {{<p>Floral initiation in mango is intricately regulated by a complex genetic network that integrates diverse environmental and endogenous cues. The flowering patterns in different varieties contingent upon their genetic network and sensitivity to the environmental signals, culminating in the phenomenon of "alternate bearing in several mango varieties. In this study, we conducted a comprehensive molecular dissection of the genetic regulators of flowering through comparative transcriptomics in mango varieties having contrast flowering pattern traits. A total of 45 and 54 million 150 base-paired reads were obtained and assembled in 58,094 and 50,945 transcripts for Amrapali and Chausa, respectively. Among them several candidates were identified as flowering regulators, operating at multilevel like floral meristem transition (SUPPRESSOR OF OVER-EXPRESSION OF CONSTANS, MiSOC1, and APETELA, MiAP1 and MiAP2), floral signal integrators (CONSTANS- LIKE, MiCOL; AGAMOUS LIKE MiAGL and SQUAMOSA PROMOTER BINDING PROTEIN LIKE, MiSPL), hormonal regulation (S-adenosyl methionine synthase, MiSAMS), cell division and cell cycle regulators (Cyclin dependant kinase, MiCDK5 and SIN-associated polypeptide, MiSAP18). Moreover, multiple isoforms of these flowering genes were also identified in these varieties. qRT-PCR analysis revealed differential transcriptional regulation of these multiple isoforms in floral and vegetative buds in the contrasting varieties. Furthermore, by investigating the gene expression of these multiple isoforms in various mango varieties with different flowering pattern traits, we established the role of these isoforms in mango flowering. Finally, gene expression findings supported by protein motif analysis and phylogenetic insights facilitated the development of a comprehensive flowering model that may applicable to subtropical ecology.</p>}},
author = {{Bajpai, Anju and M․, Muthukumar and Bajpai, Yashi and Khan, Kasim and Rajan, S. and Singh, N. K. and Singh, V. K.}},
issn = {{0304-4238}},
keywords = {{Floral induction; Floral meristem identity genes; Floral signal integrators; Isoforms diversity; Mango}},
language = {{eng}},
month = {{08}},
publisher = {{Elsevier}},
series = {{Scientia Horticulturae}},
title = {{Isoforms diversity and transcriptional regulation of floral meristem identity and pathway integrators, driving floral transition in mango under subtropics}},
url = {{http://dx.doi.org/10.1016/j.scienta.2024.113301}},
doi = {{10.1016/j.scienta.2024.113301}},
volume = {{334}},
year = {{2024}},
}