Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Modulation of the measuring range of a radioimmunoassay using an organic water two phase system

Hedenfalk, M. ; Adlercreutz, P. LU orcid and Mattiasson, B. LU (1997) In Analytica Chimica Acta 341(2-3). p.269-274
Abstract

A commercially available radioimmunoassay (RIA) kit was used to study the effects of organic solvents on antigen-antibody interactions. The RIA analysis was carried out in aqueous-organic two phase systems. After exposure to hydrophobic organic solvents the antibodies retained full binding capacity, while less hydrophobic solvents caused partial inactivation of the antibodies. A practical analysis for digoxigenin in organic solvents was developed using the RIA kit with polyclonal antibodies against digoxin. The sensitivity was modulated four orders of magnitude by the choice of organic solvent. The main factor influencing the sensitivity was the partitioning of digoxigenin between the aqueous and the organic phases. The technique... (More)

A commercially available radioimmunoassay (RIA) kit was used to study the effects of organic solvents on antigen-antibody interactions. The RIA analysis was carried out in aqueous-organic two phase systems. After exposure to hydrophobic organic solvents the antibodies retained full binding capacity, while less hydrophobic solvents caused partial inactivation of the antibodies. A practical analysis for digoxigenin in organic solvents was developed using the RIA kit with polyclonal antibodies against digoxin. The sensitivity was modulated four orders of magnitude by the choice of organic solvent. The main factor influencing the sensitivity was the partitioning of digoxigenin between the aqueous and the organic phases. The technique developed is promising for the analysis of a variety of antigens dissolved in organic solvents.

(Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antigen-antibody interactions, Immunoassay, Two phase systems
in
Analytica Chimica Acta
volume
341
issue
2-3
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:0030992428
ISSN
0003-2670
DOI
10.1016/S0003-2670(96)00448-5
language
English
LU publication?
yes
id
16c53676-c529-429c-a855-6d50b4cb1962
date added to LUP
2019-06-22 08:56:45
date last changed
2022-01-31 22:18:37
@article{16c53676-c529-429c-a855-6d50b4cb1962,
  abstract     = {{<p>A commercially available radioimmunoassay (RIA) kit was used to study the effects of organic solvents on antigen-antibody interactions. The RIA analysis was carried out in aqueous-organic two phase systems. After exposure to hydrophobic organic solvents the antibodies retained full binding capacity, while less hydrophobic solvents caused partial inactivation of the antibodies. A practical analysis for digoxigenin in organic solvents was developed using the RIA kit with polyclonal antibodies against digoxin. The sensitivity was modulated four orders of magnitude by the choice of organic solvent. The main factor influencing the sensitivity was the partitioning of digoxigenin between the aqueous and the organic phases. The technique developed is promising for the analysis of a variety of antigens dissolved in organic solvents.</p>}},
  author       = {{Hedenfalk, M. and Adlercreutz, P. and Mattiasson, B.}},
  issn         = {{0003-2670}},
  keywords     = {{Antigen-antibody interactions; Immunoassay; Two phase systems}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{2-3}},
  pages        = {{269--274}},
  publisher    = {{Elsevier}},
  series       = {{Analytica Chimica Acta}},
  title        = {{Modulation of the measuring range of a radioimmunoassay using an organic water two phase system}},
  url          = {{http://dx.doi.org/10.1016/S0003-2670(96)00448-5}},
  doi          = {{10.1016/S0003-2670(96)00448-5}},
  volume       = {{341}},
  year         = {{1997}},
}