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Transient and sustained increases in glomerular permeability following ANP infusion in rats.

Axelsson, Josefin LU ; Rippe, Anna LU and Rippe, Bengt LU (2011) In American Journal of Physiology-Renal Physiology 300. p.24-30
Abstract
The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anaesthetized Wistar rats (250-280g) the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused i.v. with ANP, 30 ng/min/kg (Lo-ANP; n=8) or 800 ng/min/kg (Hi-ANP; n=10) or 0.9% NaCl (SHAM; n=16), respectively, and with polydisperse fluorescein isothiocyanate (FITC)-Ficoll-70/400 (mol.radius 13-90Å) and (51)Cr-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60 and 120 min of ANP infusion, and analyzed by high performance size exclusion chromatography (HPLC) for determination of... (More)
The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anaesthetized Wistar rats (250-280g) the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused i.v. with ANP, 30 ng/min/kg (Lo-ANP; n=8) or 800 ng/min/kg (Hi-ANP; n=10) or 0.9% NaCl (SHAM; n=16), respectively, and with polydisperse fluorescein isothiocyanate (FITC)-Ficoll-70/400 (mol.radius 13-90Å) and (51)Cr-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60 and 120 min of ANP infusion, and analyzed by high performance size exclusion chromatography (HPLC) for determination of glomerular sieving coefficients () for Ficoll. GFR was also assessed ((51)Cr-EDTA). In Hi-ANP there was a rapid (within 5 min), but bimodal, increase in glomerular permeability. to high MW Ficoll thus reached a maximum at 15 min, after which returned to near control at 30 min, to again increase moderately at 60 and 120 min. In Lo-ANP there was also a rapid, reversible increase in glomerular , returning to near control at 30 min, followed by just a tendency of a sustained increase in permeability, but with a significant increase in "large pore" radius. In conclusion, in Hi-ANP there was a rapid increase in glomerular permeability, with an early, partly reversible permeability peak, followed by a (moderate) sustained increase in permeability. In Lo-ANP animals, only the initial permeability peak was evident. In both Lo-ANP and Hi-ANP the glomerular sieving pattern observed was found to mainly reflect an increase in the number and radius of "large pores" in the glomerular filter. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Physiology-Renal Physiology
volume
300
pages
24 - 30
publisher
American Physiological Society
external identifiers
  • wos:000285964000003
  • pmid:20943768
  • scopus:78651300320
ISSN
1522-1466
DOI
10.1152/ajprenal.00347.2010
language
English
LU publication?
yes
id
e9b65ba2-3cb4-4c1c-937d-caf608f78e5f (old id 1711205)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20943768?dopt=Abstract
date added to LUP
2010-11-05 15:51:55
date last changed
2017-08-20 04:37:51
@article{e9b65ba2-3cb4-4c1c-937d-caf608f78e5f,
  abstract     = {The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anaesthetized Wistar rats (250-280g) the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused i.v. with ANP, 30 ng/min/kg (Lo-ANP; n=8) or 800 ng/min/kg (Hi-ANP; n=10) or 0.9% NaCl (SHAM; n=16), respectively, and with polydisperse fluorescein isothiocyanate (FITC)-Ficoll-70/400 (mol.radius 13-90Å) and (51)Cr-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60 and 120 min of ANP infusion, and analyzed by high performance size exclusion chromatography (HPLC) for determination of glomerular sieving coefficients () for Ficoll. GFR was also assessed ((51)Cr-EDTA). In Hi-ANP there was a rapid (within 5 min), but bimodal, increase in glomerular permeability. to high MW Ficoll thus reached a maximum at 15 min, after which returned to near control at 30 min, to again increase moderately at 60 and 120 min. In Lo-ANP there was also a rapid, reversible increase in glomerular , returning to near control at 30 min, followed by just a tendency of a sustained increase in permeability, but with a significant increase in "large pore" radius. In conclusion, in Hi-ANP there was a rapid increase in glomerular permeability, with an early, partly reversible permeability peak, followed by a (moderate) sustained increase in permeability. In Lo-ANP animals, only the initial permeability peak was evident. In both Lo-ANP and Hi-ANP the glomerular sieving pattern observed was found to mainly reflect an increase in the number and radius of "large pores" in the glomerular filter.},
  author       = {Axelsson, Josefin and Rippe, Anna and Rippe, Bengt},
  issn         = {1522-1466},
  language     = {eng},
  pages        = {24--30},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology-Renal Physiology},
  title        = {Transient and sustained increases in glomerular permeability following ANP infusion in rats.},
  url          = {http://dx.doi.org/10.1152/ajprenal.00347.2010},
  volume       = {300},
  year         = {2011},
}