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Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis.

Karagin, Peren H; Stenram, Unne LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2010) In World Journal of Gastroenterology 16(38). p.4817-4822
Abstract
AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter... (More)
AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
World Journal of Gastroenterology
volume
16
issue
38
pages
4817 - 4822
publisher
WJG Press
external identifiers
  • wos:000283185800008
  • pmid:20939110
  • scopus:78049487203
ISSN
1007-9327
DOI
10.3748/wjg.v16.i38.4817
language
English
LU publication?
yes
id
c9af5984-3bf8-4c82-b4d4-0dd2006cd51c (old id 1711284)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20939110?dopt=Abstract
date added to LUP
2010-11-05 12:38:50
date last changed
2018-05-29 11:40:34
@article{c9af5984-3bf8-4c82-b4d4-0dd2006cd51c,
  abstract     = {AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.},
  author       = {Karagin, Peren H and Stenram, Unne and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1007-9327},
  language     = {eng},
  number       = {38},
  pages        = {4817--4822},
  publisher    = {WJG Press},
  series       = {World Journal of Gastroenterology},
  title        = {Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis.},
  url          = {http://dx.doi.org/10.3748/wjg.v16.i38.4817},
  volume       = {16},
  year         = {2010},
}