Anti-CD44 Monoclonal Antibody Inhibits Heart Transplant Rejection Mediated by Alloantigen-primed CD4(+) Memory T Cells in Nude Mice
(2010) In Immunological Investigations 39(8). p.807-819- Abstract
- Donor-reactive CD4(+) memory T cells threaten the survival of transplanted organs. In this study, we used anti-CD44 monoclonal antibody (mAb) to inhibit adoptively transferred B6-reactive CD4(+) memory T cells (BALB/c origin) and to induce tolerance of B6 hearts in nude mice. The median survival time (MST) of the grafts was 6 days in the isotype group, and more than 100 days in the group treated with 8 doses of anti-CD44 at four-day intervals. Histological analysis revealed that the mean rejection level was Grade 3 in the isotype group, and Grade 0 or 1 in the multi-dose anti-CD44 treatment group. Compared with the isotype group, the multiply treated anti-CD44 group had significantly decreased IL-2 and IFN-gamma expressions, while IL-10... (More)
- Donor-reactive CD4(+) memory T cells threaten the survival of transplanted organs. In this study, we used anti-CD44 monoclonal antibody (mAb) to inhibit adoptively transferred B6-reactive CD4(+) memory T cells (BALB/c origin) and to induce tolerance of B6 hearts in nude mice. The median survival time (MST) of the grafts was 6 days in the isotype group, and more than 100 days in the group treated with 8 doses of anti-CD44 at four-day intervals. Histological analysis revealed that the mean rejection level was Grade 3 in the isotype group, and Grade 0 or 1 in the multi-dose anti-CD44 treatment group. Compared with the isotype group, the multiply treated anti-CD44 group had significantly decreased IL-2 and IFN-gamma expressions, while IL-10 and TGF-beta were increased in the serum and the graft. Foxp3 in the graft was also increased. These data demonstrate that alloreactive CD4(+) memory T cells mediate the destruction of allografts, and the adhesion molecule CD44 plays an important role in this course. Anti-CD44 mAb may promote the reduction of CD4(+) memory T cells and the production of regulatory T cells (Tregs). Furthermore, Tregs are maintained at a certain level while suppressing cellular immunity and inducing the grafts long-term survival in transplant recipients. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1720845
- author
- Wang, Feng ; Chen, Jibing ; Shao, Wei ; Xie, Baiyi ; Wang, Yongzhi ; Lan, Tianshu ; Thorlacius, Henrik LU and Qi, Zhongquan
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cardiac transplantation, Anti-CD44 mAb, CD4(+) memory T cells
- in
- Immunological Investigations
- volume
- 39
- issue
- 8
- pages
- 807 - 819
- publisher
- Informa Healthcare
- external identifiers
-
- wos:000283076800003
- scopus:77958165118
- pmid:20718664
- ISSN
- 0882-0139
- DOI
- 10.3109/08820139.2010.497833
- language
- English
- LU publication?
- yes
- id
- db89ce13-62c4-4ff9-b490-8d09b1ee342b (old id 1720845)
- date added to LUP
- 2016-04-01 14:29:43
- date last changed
- 2025-02-01 06:40:29
@article{db89ce13-62c4-4ff9-b490-8d09b1ee342b,
abstract = {{Donor-reactive CD4(+) memory T cells threaten the survival of transplanted organs. In this study, we used anti-CD44 monoclonal antibody (mAb) to inhibit adoptively transferred B6-reactive CD4(+) memory T cells (BALB/c origin) and to induce tolerance of B6 hearts in nude mice. The median survival time (MST) of the grafts was 6 days in the isotype group, and more than 100 days in the group treated with 8 doses of anti-CD44 at four-day intervals. Histological analysis revealed that the mean rejection level was Grade 3 in the isotype group, and Grade 0 or 1 in the multi-dose anti-CD44 treatment group. Compared with the isotype group, the multiply treated anti-CD44 group had significantly decreased IL-2 and IFN-gamma expressions, while IL-10 and TGF-beta were increased in the serum and the graft. Foxp3 in the graft was also increased. These data demonstrate that alloreactive CD4(+) memory T cells mediate the destruction of allografts, and the adhesion molecule CD44 plays an important role in this course. Anti-CD44 mAb may promote the reduction of CD4(+) memory T cells and the production of regulatory T cells (Tregs). Furthermore, Tregs are maintained at a certain level while suppressing cellular immunity and inducing the grafts long-term survival in transplant recipients.}},
author = {{Wang, Feng and Chen, Jibing and Shao, Wei and Xie, Baiyi and Wang, Yongzhi and Lan, Tianshu and Thorlacius, Henrik and Qi, Zhongquan}},
issn = {{0882-0139}},
keywords = {{Cardiac transplantation; Anti-CD44 mAb; CD4(+) memory T cells}},
language = {{eng}},
number = {{8}},
pages = {{807--819}},
publisher = {{Informa Healthcare}},
series = {{Immunological Investigations}},
title = {{Anti-CD44 Monoclonal Antibody Inhibits Heart Transplant Rejection Mediated by Alloantigen-primed CD4(+) Memory T Cells in Nude Mice}},
url = {{http://dx.doi.org/10.3109/08820139.2010.497833}},
doi = {{10.3109/08820139.2010.497833}},
volume = {{39}},
year = {{2010}},
}