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Enzymatic enrichment of omega-3 polyunsaturated fatty acids in Nile perch (Lates niloticus) viscera oil

Mbatia, Betty LU ; Adlercreutz, Patrick LU ; Mulaa, Francis and Mattiasson, Bo LU (2010) In European Journal of Lipid Science and Technology 112(9). p.977-984
Abstract
Oil was extracted from fatty material obtained from Nile perch viscera using the protease Protex 30L. Enrichment of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the glyceride fraction was carried out by hydrolysis of extracted oils with lipases from Candida rugosa, Thermomyces lanuginosus and Pseudomonas cepacia. The unusual fatty acid distribution of the oil influenced the apparent lipase specificity to a large extent. In the unhydrolysed oil, only 16% of EPA was in sn-2 position while 51% of palmitic acid was located in this position of the triacylglycerol (TAG) molecules. Non-regioselective lipase from C. rugosa was the most effective in combined enrichment of both EPA and DHA. This was partly because it was able to... (More)
Oil was extracted from fatty material obtained from Nile perch viscera using the protease Protex 30L. Enrichment of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the glyceride fraction was carried out by hydrolysis of extracted oils with lipases from Candida rugosa, Thermomyces lanuginosus and Pseudomonas cepacia. The unusual fatty acid distribution of the oil influenced the apparent lipase specificity to a large extent. In the unhydrolysed oil, only 16% of EPA was in sn-2 position while 51% of palmitic acid was located in this position of the triacylglycerol (TAG) molecules. Non-regioselective lipase from C. rugosa was the most effective in combined enrichment of both EPA and DHA. This was partly because it was able to hydrolyse off palmitic acid from the sn-2 position, which 1-, 3-specific lipases were unable to do. Hydrolysis with C. rugosa lipase enriched EPA from 3 to 6 mol% and DHA from 9 to 23 mol%, with recoveries of 42 and 55%, respectively. The 1-, 3-specific lipase from T. lanuginosus was ineffective in enriching EPA, but gave best DHA enrichment, 38 mol% with a recovery of 39%. DHA was rather equally distributed in sn-1, - 2 and - 3 positions of TAG. The results show that both the fatty acid specificity and regiospecificity of the lipase as well as the fatty acid distribution of the oil should be considered when choosing the strategy for fatty acid enrichment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Specificity, constant, Nile perch oil, Lipase-catalysed hydrolysis, DHA, EPA
in
European Journal of Lipid Science and Technology
volume
112
issue
9
pages
977 - 984
publisher
John Wiley & Sons
external identifiers
  • wos:000282662800007
  • scopus:77956928560
ISSN
1438-7697
DOI
10.1002/ejlt.201000009
language
English
LU publication?
yes
id
cb11555f-bf32-47a4-a715-910eb54e2be7 (old id 1720920)
date added to LUP
2010-12-03 13:19:57
date last changed
2018-05-29 10:20:46
@article{cb11555f-bf32-47a4-a715-910eb54e2be7,
  abstract     = {Oil was extracted from fatty material obtained from Nile perch viscera using the protease Protex 30L. Enrichment of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the glyceride fraction was carried out by hydrolysis of extracted oils with lipases from Candida rugosa, Thermomyces lanuginosus and Pseudomonas cepacia. The unusual fatty acid distribution of the oil influenced the apparent lipase specificity to a large extent. In the unhydrolysed oil, only 16% of EPA was in sn-2 position while 51% of palmitic acid was located in this position of the triacylglycerol (TAG) molecules. Non-regioselective lipase from C. rugosa was the most effective in combined enrichment of both EPA and DHA. This was partly because it was able to hydrolyse off palmitic acid from the sn-2 position, which 1-, 3-specific lipases were unable to do. Hydrolysis with C. rugosa lipase enriched EPA from 3 to 6 mol% and DHA from 9 to 23 mol%, with recoveries of 42 and 55%, respectively. The 1-, 3-specific lipase from T. lanuginosus was ineffective in enriching EPA, but gave best DHA enrichment, 38 mol% with a recovery of 39%. DHA was rather equally distributed in sn-1, - 2 and - 3 positions of TAG. The results show that both the fatty acid specificity and regiospecificity of the lipase as well as the fatty acid distribution of the oil should be considered when choosing the strategy for fatty acid enrichment.},
  author       = {Mbatia, Betty and Adlercreutz, Patrick and Mulaa, Francis and Mattiasson, Bo},
  issn         = {1438-7697},
  keyword      = {Specificity,constant,Nile perch oil,Lipase-catalysed hydrolysis,DHA,EPA},
  language     = {eng},
  number       = {9},
  pages        = {977--984},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Lipid Science and Technology},
  title        = {Enzymatic enrichment of omega-3 polyunsaturated fatty acids in Nile perch (Lates niloticus) viscera oil},
  url          = {http://dx.doi.org/10.1002/ejlt.201000009},
  volume       = {112},
  year         = {2010},
}