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Hordein variation in the genus Hordeum as recognized by monoclonal antibodies.

Pelger, Susanne LU and von Bothmer, Roland (1992) In Genome 35(2). p.200-207
Abstract
Abstract: The composition of the major storage protein, hordein, in wild barley species has been studied by using gel electrophoresis, Coomassie staining, and immunoblot assays. We have shown earlier that it is possible to obtain cross-reaction outside the cultivated barley, with monoclonal antibodies raised against hordeins from the barley cultivar Bomi. These antibodies have now been used to investigate the hordein composition in all species of the Hordeum genus. The results showed that polypeptides similar to the two major hordein groups of cultivated barley, the B- and C-hordeins, are produced in all wild Hordeum species, and that there are both similarities and differences between the two hordein groups. The similarities indicate a... (More)
Abstract: The composition of the major storage protein, hordein, in wild barley species has been studied by using gel electrophoresis, Coomassie staining, and immunoblot assays. We have shown earlier that it is possible to obtain cross-reaction outside the cultivated barley, with monoclonal antibodies raised against hordeins from the barley cultivar Bomi. These antibodies have now been used to investigate the hordein composition in all species of the Hordeum genus. The results showed that polypeptides similar to the two major hordein groups of cultivated barley, the B- and C-hordeins, are produced in all wild Hordeum species, and that there are both similarities and differences between the two hordein groups. The similarities indicate a common evolutionary origin, while the distinction between B- and C-hordeins in the entire genus clearly shows that the divergence of their coding genes preceded the divergence of the Hordeum species. The presence of the same antigenic site in two different species indicates that they are evolutionarily related. Among the wild species, two rarely occurring sites were exclusively found in H. vulgare ssp. spontaneum and H. bulbosum, which confirms that they are the cultivated barley's closest relatives. Some of the antibodies also gave an extensive reaction pattern with H. murinum, which suggests a fairly close relationship to H vulgare, though not as close as between H. vulgare and H. bulbosum. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Genome
volume
35
issue
2
pages
200 - 207
publisher
NRC Research Press
external identifiers
  • scopus:0026748797
ISSN
0831-2796
DOI
10.1139/g92-031
language
English
LU publication?
yes
id
b8d1902f-541d-4494-bcbd-81423d049d8a (old id 1729307)
date added to LUP
2010-11-23 17:41:17
date last changed
2017-01-01 04:23:32
@article{b8d1902f-541d-4494-bcbd-81423d049d8a,
  abstract     = {Abstract: The composition of the major storage protein, hordein, in wild barley species has been studied by using gel electrophoresis, Coomassie staining, and immunoblot assays. We have shown earlier that it is possible to obtain cross-reaction outside the cultivated barley, with monoclonal antibodies raised against hordeins from the barley cultivar Bomi. These antibodies have now been used to investigate the hordein composition in all species of the Hordeum genus. The results showed that polypeptides similar to the two major hordein groups of cultivated barley, the B- and C-hordeins, are produced in all wild Hordeum species, and that there are both similarities and differences between the two hordein groups. The similarities indicate a common evolutionary origin, while the distinction between B- and C-hordeins in the entire genus clearly shows that the divergence of their coding genes preceded the divergence of the Hordeum species. The presence of the same antigenic site in two different species indicates that they are evolutionarily related. Among the wild species, two rarely occurring sites were exclusively found in H. vulgare ssp. spontaneum and H. bulbosum, which confirms that they are the cultivated barley's closest relatives. Some of the antibodies also gave an extensive reaction pattern with H. murinum, which suggests a fairly close relationship to H vulgare, though not as close as between H. vulgare and H. bulbosum.},
  author       = {Pelger, Susanne and von Bothmer, Roland},
  issn         = {0831-2796},
  language     = {eng},
  number       = {2},
  pages        = {200--207},
  publisher    = {NRC Research Press},
  series       = {Genome},
  title        = {Hordein variation in the genus Hordeum as recognized by monoclonal antibodies.},
  url          = {http://dx.doi.org/10.1139/g92-031},
  volume       = {35},
  year         = {1992},
}