Hordein variation in the genus Hordeum as recognized by monoclonal antibodies.
(1992) In Genome 35(2). p.200-207- Abstract
- Abstract: The composition of the major storage protein, hordein, in wild barley species has been studied by using gel electrophoresis, Coomassie staining, and immunoblot assays. We have shown earlier that it is possible to obtain cross-reaction outside the cultivated barley, with monoclonal antibodies raised against hordeins from the barley cultivar Bomi. These antibodies have now been used to investigate the hordein composition in all species of the Hordeum genus. The results showed that polypeptides similar to the two major hordein groups of cultivated barley, the B- and C-hordeins, are produced in all wild Hordeum species, and that there are both similarities and differences between the two hordein groups. The similarities indicate a... (More)
- Abstract: The composition of the major storage protein, hordein, in wild barley species has been studied by using gel electrophoresis, Coomassie staining, and immunoblot assays. We have shown earlier that it is possible to obtain cross-reaction outside the cultivated barley, with monoclonal antibodies raised against hordeins from the barley cultivar Bomi. These antibodies have now been used to investigate the hordein composition in all species of the Hordeum genus. The results showed that polypeptides similar to the two major hordein groups of cultivated barley, the B- and C-hordeins, are produced in all wild Hordeum species, and that there are both similarities and differences between the two hordein groups. The similarities indicate a common evolutionary origin, while the distinction between B- and C-hordeins in the entire genus clearly shows that the divergence of their coding genes preceded the divergence of the Hordeum species. The presence of the same antigenic site in two different species indicates that they are evolutionarily related. Among the wild species, two rarely occurring sites were exclusively found in H. vulgare ssp. spontaneum and H. bulbosum, which confirms that they are the cultivated barley's closest relatives. Some of the antibodies also gave an extensive reaction pattern with H. murinum, which suggests a fairly close relationship to H vulgare, though not as close as between H. vulgare and H. bulbosum. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1729307
- author
- Pelger, Susanne LU and von Bothmer, Roland
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genome
- volume
- 35
- issue
- 2
- pages
- 200 - 207
- publisher
- Canadian Science Publishing, NRC Research Press
- external identifiers
-
- scopus:0026748797
- ISSN
- 0831-2796
- DOI
- 10.1139/g92-031
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Science (000006100)
- id
- b8d1902f-541d-4494-bcbd-81423d049d8a (old id 1729307)
- date added to LUP
- 2016-04-01 11:38:06
- date last changed
- 2021-02-21 03:21:19
@article{b8d1902f-541d-4494-bcbd-81423d049d8a,
abstract = {{Abstract: The composition of the major storage protein, hordein, in wild barley species has been studied by using gel electrophoresis, Coomassie staining, and immunoblot assays. We have shown earlier that it is possible to obtain cross-reaction outside the cultivated barley, with monoclonal antibodies raised against hordeins from the barley cultivar Bomi. These antibodies have now been used to investigate the hordein composition in all species of the Hordeum genus. The results showed that polypeptides similar to the two major hordein groups of cultivated barley, the B- and C-hordeins, are produced in all wild Hordeum species, and that there are both similarities and differences between the two hordein groups. The similarities indicate a common evolutionary origin, while the distinction between B- and C-hordeins in the entire genus clearly shows that the divergence of their coding genes preceded the divergence of the Hordeum species. The presence of the same antigenic site in two different species indicates that they are evolutionarily related. Among the wild species, two rarely occurring sites were exclusively found in H. vulgare ssp. spontaneum and H. bulbosum, which confirms that they are the cultivated barley's closest relatives. Some of the antibodies also gave an extensive reaction pattern with H. murinum, which suggests a fairly close relationship to H vulgare, though not as close as between H. vulgare and H. bulbosum.}},
author = {{Pelger, Susanne and von Bothmer, Roland}},
issn = {{0831-2796}},
language = {{eng}},
number = {{2}},
pages = {{200--207}},
publisher = {{Canadian Science Publishing, NRC Research Press}},
series = {{Genome}},
title = {{Hordein variation in the genus Hordeum as recognized by monoclonal antibodies.}},
url = {{http://dx.doi.org/10.1139/g92-031}},
doi = {{10.1139/g92-031}},
volume = {{35}},
year = {{1992}},
}