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The reaction of monoclonal antibodies with hordeins from five different Hordeum species.

Pelger, Susanne LU and Höyer-Hansen, Gunilla (1989) In Hereditas 111(3). p.273-279
Abstract
Hordeins from H. vulgare, H. murinum, H. pusillum, H. bulbosum, and H. brevisubulatum were probed with five monoclonal antibodies raised against different hordein polypeptides from Hordeum vulgare cv. ‘Bomi’. The response pattern differed widely among species. One of the antibodies, CMpBChorl, was found to react with hordein from every tested species, and all of the antibodies reacted with at least one of the wild barley species. Antibodies reacting with many different hordein polypeptides in ‘Bomi’ showed a wide reaction also with other species, while more specific antibodies gave limited reactions outside of domesticated barley. In some cases hordein polypeptides reacting with a specific antibody differed in electrophoretic mobility... (More)
Hordeins from H. vulgare, H. murinum, H. pusillum, H. bulbosum, and H. brevisubulatum were probed with five monoclonal antibodies raised against different hordein polypeptides from Hordeum vulgare cv. ‘Bomi’. The response pattern differed widely among species. One of the antibodies, CMpBChorl, was found to react with hordein from every tested species, and all of the antibodies reacted with at least one of the wild barley species. Antibodies reacting with many different hordein polypeptides in ‘Bomi’ showed a wide reaction also with other species, while more specific antibodies gave limited reactions outside of domesticated barley. In some cases hordein polypeptides reacting with a specific antibody differed in electrophoretic mobility between species, indicating that the hordein genes involved have undergone concerted evolution. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Hereditas
volume
111
issue
3
pages
273 - 279
publisher
Wiley-Blackwell
external identifiers
  • scopus:0024892344
ISSN
1601-5223
DOI
10.1111/j.1601-5223.1990.tb00406.x
language
English
LU publication?
yes
id
44c2d38c-696b-4edb-8676-01d863e254f4 (old id 1729314)
date added to LUP
2010-11-23 17:43:52
date last changed
2017-01-01 04:34:31
@article{44c2d38c-696b-4edb-8676-01d863e254f4,
  abstract     = {Hordeins from H. vulgare, H. murinum, H. pusillum, H. bulbosum, and H. brevisubulatum were probed with five monoclonal antibodies raised against different hordein polypeptides from Hordeum vulgare cv. ‘Bomi’. The response pattern differed widely among species. One of the antibodies, CMpBChorl, was found to react with hordein from every tested species, and all of the antibodies reacted with at least one of the wild barley species. Antibodies reacting with many different hordein polypeptides in ‘Bomi’ showed a wide reaction also with other species, while more specific antibodies gave limited reactions outside of domesticated barley. In some cases hordein polypeptides reacting with a specific antibody differed in electrophoretic mobility between species, indicating that the hordein genes involved have undergone concerted evolution.},
  author       = {Pelger, Susanne and Höyer-Hansen, Gunilla},
  issn         = {1601-5223},
  language     = {eng},
  number       = {3},
  pages        = {273--279},
  publisher    = {Wiley-Blackwell},
  series       = {Hereditas},
  title        = {The reaction of monoclonal antibodies with hordeins from five different Hordeum species.},
  url          = {http://dx.doi.org/10.1111/j.1601-5223.1990.tb00406.x},
  volume       = {111},
  year         = {1989},
}