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Crypt fission contributes to postnatal epithelial growth of the small intestine in pigs

Slupecka, M.; Wolinski, J. and Pierzynowski, Stefan LU (2010) 11th International Symposium on Digestive Physiology of Pigs In Livestock Science 133(1-3). p.34-37
Abstract
Studies on growth of the small intestine have mostly concentrated on crypt hyperplasia rather than crypt fission (CF). The process of crypt bifurcation has been identified in rodents and humans but not in swine. The main aims of this study were: 1) to investigate quantitatively crypt fission in the small intestine mucosa of pigs of different age-groups and 2) to assess the effect of colostrum and milk on small intestine growth via CF. Five groups of piglets were studied: non-suckling piglets, euthanized just after delivery (C0) suckling piglets, euthanized 24 h after delivery (C1) and then piglets that were euthanized at 7.28 and 180 days of life (C7, C28, C180 respectively). Samples of duodenum, jejunum (proximal, middle and distal) and... (More)
Studies on growth of the small intestine have mostly concentrated on crypt hyperplasia rather than crypt fission (CF). The process of crypt bifurcation has been identified in rodents and humans but not in swine. The main aims of this study were: 1) to investigate quantitatively crypt fission in the small intestine mucosa of pigs of different age-groups and 2) to assess the effect of colostrum and milk on small intestine growth via CF. Five groups of piglets were studied: non-suckling piglets, euthanized just after delivery (C0) suckling piglets, euthanized 24 h after delivery (C1) and then piglets that were euthanized at 7.28 and 180 days of life (C7, C28, C180 respectively). Samples of duodenum, jejunum (proximal, middle and distal) and ileum were taken for the evaluation of the percentage of bifid crypts. Our results show that the 24 h suckling period has no effect on the percentage of bifid crypts compared to the non-suckling, newborn piglets (C0). Moreover, we observed that the rate of bifid crypts depends predominantly on the age of the piglets and the part of intestine examined and less on the feed components. The highest CF is observed during the first 28 days of life and then declines significantly at day 180. In duodenum and proximal jejunum there were no significant changes between groups of piglets in age till 28 days of life whereas in further parts we observed a decrease of bifid crypts comparing C0 and C1 with C7. In conclusion, our data shows that CF contributes to epithelial growth of the small intestine in piglets. The peak of bifid crypts was observed during suckling, thus CF can be described as a marker for intestinal growth during the early postnatal period. (C) 2010 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Crypt fission, Small intestine, Pigs
in
Livestock Science
volume
133
issue
1-3
pages
34 - 37
publisher
Elsevier
conference name
11th International Symposium on Digestive Physiology of Pigs
external identifiers
  • wos:000284726000008
  • scopus:77957160001
ISSN
1871-1413
DOI
10.1016/j.livsci.2010.06.012
language
English
LU publication?
yes
id
c0121696-dcbe-441e-ac6f-7338ae1d73ae (old id 1751640)
date added to LUP
2010-12-29 13:27:33
date last changed
2018-05-29 12:17:08
@inproceedings{c0121696-dcbe-441e-ac6f-7338ae1d73ae,
  abstract     = {Studies on growth of the small intestine have mostly concentrated on crypt hyperplasia rather than crypt fission (CF). The process of crypt bifurcation has been identified in rodents and humans but not in swine. The main aims of this study were: 1) to investigate quantitatively crypt fission in the small intestine mucosa of pigs of different age-groups and 2) to assess the effect of colostrum and milk on small intestine growth via CF. Five groups of piglets were studied: non-suckling piglets, euthanized just after delivery (C0) suckling piglets, euthanized 24 h after delivery (C1) and then piglets that were euthanized at 7.28 and 180 days of life (C7, C28, C180 respectively). Samples of duodenum, jejunum (proximal, middle and distal) and ileum were taken for the evaluation of the percentage of bifid crypts. Our results show that the 24 h suckling period has no effect on the percentage of bifid crypts compared to the non-suckling, newborn piglets (C0). Moreover, we observed that the rate of bifid crypts depends predominantly on the age of the piglets and the part of intestine examined and less on the feed components. The highest CF is observed during the first 28 days of life and then declines significantly at day 180. In duodenum and proximal jejunum there were no significant changes between groups of piglets in age till 28 days of life whereas in further parts we observed a decrease of bifid crypts comparing C0 and C1 with C7. In conclusion, our data shows that CF contributes to epithelial growth of the small intestine in piglets. The peak of bifid crypts was observed during suckling, thus CF can be described as a marker for intestinal growth during the early postnatal period. (C) 2010 Elsevier B.V. All rights reserved.},
  author       = {Slupecka, M. and Wolinski, J. and Pierzynowski, Stefan},
  booktitle    = {Livestock Science},
  issn         = {1871-1413},
  keyword      = {Crypt fission,Small intestine,Pigs},
  language     = {eng},
  number       = {1-3},
  pages        = {34--37},
  publisher    = {Elsevier},
  title        = {Crypt fission contributes to postnatal epithelial growth of the small intestine in pigs},
  url          = {http://dx.doi.org/10.1016/j.livsci.2010.06.012},
  volume       = {133},
  year         = {2010},
}