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Small Islets are Essential for Successful Intraportal Transplantation in a Diabetes Mouse Model

Su, Z.; Xia, J.; Shao, W.; Cui, Y.; Tai, S.; Ekberg, Henrik LU ; Corbascio, M.; Chen, J. and Qi, Z. (2010) In Scandinavian Journal of Immunology 72(6). p.504-510
Abstract
Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter < 250 mu m, group A) or large (average diameter > 250 mu m, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in... (More)
Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter < 250 mu m, group A) or large (average diameter > 250 mu m, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in both groups, but the pathology in group B was significantly worse. Multiple proinflammatory cytokines were significantly higher in group B than that of A at 3 h post-transplantation. Our study determined that the size of islets plays a critical role in the success of intraportal islet transplantation (IPIT) and should be taken into account in future IPIT protocols for the treatment of diabetes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Immunology
volume
72
issue
6
pages
504 - 510
publisher
Wiley-Blackwell
external identifiers
  • wos:000283993100004
  • scopus:78149257689
ISSN
1365-3083
DOI
10.1111/j.1365-3083.2010.02466.x
language
English
LU publication?
yes
id
04b2fde5-fa06-4ad0-a098-16443609739f (old id 1752312)
date added to LUP
2011-01-04 07:34:56
date last changed
2018-05-29 10:18:22
@article{04b2fde5-fa06-4ad0-a098-16443609739f,
  abstract     = {Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter &lt; 250 mu m, group A) or large (average diameter &gt; 250 mu m, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in both groups, but the pathology in group B was significantly worse. Multiple proinflammatory cytokines were significantly higher in group B than that of A at 3 h post-transplantation. Our study determined that the size of islets plays a critical role in the success of intraportal islet transplantation (IPIT) and should be taken into account in future IPIT protocols for the treatment of diabetes.},
  author       = {Su, Z. and Xia, J. and Shao, W. and Cui, Y. and Tai, S. and Ekberg, Henrik and Corbascio, M. and Chen, J. and Qi, Z.},
  issn         = {1365-3083},
  language     = {eng},
  number       = {6},
  pages        = {504--510},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Small Islets are Essential for Successful Intraportal Transplantation in a Diabetes Mouse Model},
  url          = {http://dx.doi.org/10.1111/j.1365-3083.2010.02466.x},
  volume       = {72},
  year         = {2010},
}