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Tissue proteome profiling of preeclamptic placenta using recombinant antibody microarrays

Dexlin Mellby, Linda LU ; Sandström Gerdtsson, Anna LU ; Centlow, Magnus LU ; Sjögren, Sara LU ; Hansson, Stefan LU ; Borrebaeck, Carl LU and Wingren, Christer LU (2010) In Proteomics Clinical Applications 4(10-11). p.794-807
Abstract
Purpose: Preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE. Experimental design: We used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as... (More)
Purpose: Preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE. Experimental design: We used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions. Results: The data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values <= 0.91) could be distinguished. Notably, the data implied that all subgroups, but preeclampsia with bilateral notching and IUGR, could be further classified into novel subsets (ROC AUC values of 1.0) displaying different inflammatory signatures. Conclusions and clinical relevance: We have taken one step further toward de-convoluting the complex features of PE at the molecular level using affinity proteomics. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Preeclampsia, Membrane proteomics, Affinity proteomics, Antibody microarrays, Protein expression profiling
in
Proteomics Clinical Applications
volume
4
issue
10-11
pages
794 - 807
publisher
John Wiley & Sons
external identifiers
  • wos:000284044300002
  • pmid:21137023
  • scopus:78349288535
ISSN
1862-8354
DOI
10.1002/prca.201000001
project
CREATE Health
language
English
LU publication?
yes
id
9b696e41-d6b5-42d0-ba93-f683ab2837be (old id 1752759)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21137023?dopt=Abstract
date added to LUP
2011-01-03 12:40:36
date last changed
2018-05-29 11:30:55
@article{9b696e41-d6b5-42d0-ba93-f683ab2837be,
  abstract     = {Purpose: Preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE. Experimental design: We used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions. Results: The data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values &lt;= 0.91) could be distinguished. Notably, the data implied that all subgroups, but preeclampsia with bilateral notching and IUGR, could be further classified into novel subsets (ROC AUC values of 1.0) displaying different inflammatory signatures. Conclusions and clinical relevance: We have taken one step further toward de-convoluting the complex features of PE at the molecular level using affinity proteomics.},
  author       = {Dexlin Mellby, Linda and Sandström Gerdtsson, Anna and Centlow, Magnus and Sjögren, Sara and Hansson, Stefan and Borrebaeck, Carl and Wingren, Christer},
  issn         = {1862-8354},
  keyword      = {Preeclampsia,Membrane proteomics,Affinity proteomics,Antibody microarrays,Protein expression profiling},
  language     = {eng},
  number       = {10-11},
  pages        = {794--807},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics Clinical Applications},
  title        = {Tissue proteome profiling of preeclamptic placenta using recombinant antibody microarrays},
  url          = {http://dx.doi.org/10.1002/prca.201000001},
  volume       = {4},
  year         = {2010},
}