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Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering.

Wahlund, Karl-Gustav LU ; Leeman, Mats LU and Santacruz, Stalin LU (2011) In Analytical and Bioanalytical Chemistry 399. p.1455-1465
Abstract
Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was... (More)
Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
399
pages
1455 - 1465
publisher
Springer
external identifiers
  • wos:000286599200007
  • pmid:21181137
  • scopus:79451475321
ISSN
1618-2642
DOI
10.1007/s00216-010-4438-5
language
English
LU publication?
yes
id
67beb767-47c2-48f0-898a-618f497a099e (old id 1755843)
date added to LUP
2011-01-11 14:10:36
date last changed
2017-07-02 03:21:32
@article{67beb767-47c2-48f0-898a-618f497a099e,
  abstract     = {Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties.},
  author       = {Wahlund, Karl-Gustav and Leeman, Mats and Santacruz, Stalin},
  issn         = {1618-2642},
  language     = {eng},
  pages        = {1455--1465},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering.},
  url          = {http://dx.doi.org/10.1007/s00216-010-4438-5},
  volume       = {399},
  year         = {2011},
}