Conserved function of the Krüppel gap gene in the blowfly Lucilia sericata, despite anterior shift of expression.
Blechert, Oliver ; Douglas, Desiree LU and Baumgartner, Stefan LU
(2011)
In Insect Molecular Biology
20.
p.257-265
- Abstract
- To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an... (More)
- To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an anterior shift in expression, Kr function has remained conserved during the evolution of the blowflies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1756223
- author
- Blechert, Oliver
; Douglas, Desiree
LU
and Baumgartner, Stefan
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Insect Molecular Biology
- volume
- 20
- pages
- 257 - 265
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000287927400010
- pmid:21166911
- scopus:79952300556
- ISSN
- 1365-2583
- DOI
- 10.1111/j.1365-2583.2010.01063.x
- project
- Formation of the bicoid gradient in Drosophila
- language
- English
- LU publication?
- yes
- id
- 5af33f0f-e3a7-4427-b201-910e559d9537 (old id 1756223)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21166911?dopt=Abstract
- date added to LUP
- 2016-04-04 09:40:11
- date last changed
- 2022-01-29 18:59:36
@article{5af33f0f-e3a7-4427-b201-910e559d9537,
abstract = {{To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an anterior shift in expression, Kr function has remained conserved during the evolution of the blowflies.}},
author = {{Blechert, Oliver and Douglas, Desiree and Baumgartner, Stefan}},
issn = {{1365-2583}},
language = {{eng}},
pages = {{257--265}},
publisher = {{Wiley-Blackwell}},
series = {{Insect Molecular Biology}},
title = {{Conserved function of the Krüppel gap gene in the blowfly Lucilia sericata, despite anterior shift of expression.}},
url = {{http://dx.doi.org/10.1111/j.1365-2583.2010.01063.x}},
doi = {{10.1111/j.1365-2583.2010.01063.x}},
volume = {{20}},
year = {{2011}},
}